globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1612322114
论文题名:
Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
作者: Andreu-Fernández V.; Sancho M.; Genovés A.; Lucendo E.; Todt F.; Lauterwasser J.; Funk K.; Jahreis G.; Pérez-Payá E.; Mingarro I.; Edlich F.; Orzáez M.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期:2
起始页码: 310
结束页码: 315
语种: 英语
英文关键词: Apoptosis ; Bcl-2 ; Mitochondria ; Oligomerization ; Transmembrane
Scopus关键词: protein Bax ; protein bcl 2 ; protein bcl xl ; apoptosis regulatory protein ; membrane protein ; protein Bax ; protein bcl x ; protein binding ; apoptosis ; bacterial membrane ; carboxy terminal sequence ; Conference Paper ; controlled study ; human ; human cell ; membrane permeability ; mitochondrial membrane ; nonhuman ; oligomerization ; outer membrane ; priority journal ; protein domain ; protein protein interaction ; cell membrane ; Escherichia coli ; HCT 116 cell line ; metabolism ; mitochondrion ; physiology ; protein domain ; tumor cell line ; Apoptosis ; Apoptosis Regulatory Proteins ; bcl-2-Associated X Protein ; bcl-X Protein ; Cell Line, Tumor ; Cell Membrane ; Escherichia coli ; HCT116 Cells ; Humans ; Membrane Proteins ; Mitochondria ; Mitochondrial Membranes ; Protein Binding ; Protein Domains
英文摘要: The Bcl-2 (B-cell lymphoma 2) protein Bax (Bcl-2 associated X, apoptosis regulator) can commit cells to apoptosis via outer mitochondrial membrane permeabilization. Bax activity is controlled in healthy cells by prosurvival Bcl-2 proteins. C-terminal Bax transmembrane domain interactions were implicated recently in Bax pore formation. Here, we show that the isolated transmembrane domains of Bax, Bcl-xL (B-cell lymphoma-extra large), and Bcl-2 can mediate interactions between Bax and prosurvival proteins inside the membrane in the absence of apoptotic stimuli. Bcl-2 protein transmembrane domains specifically homooligomerize and heterooligomerize in bacterial and mitochondrial membranes. Their interactions participate in the regulation of Bcl-2 proteins, thus modulating apoptotic activity. Our results suggest that interactions between the transmembrane domains of Bax and antiapoptotic Bcl-2 proteins represent a previously unappreciated level of apoptosis regulation.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163883
Appears in Collections:气候变化与战略

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作者单位: Andreu-Fernández, V., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain, Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, Departament de Bioquímica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina, Universitat de València, Burjassot, 46100, Spain; Sancho, M., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Genovés, A., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Lucendo, E., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain; Todt, F., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany; Lauterwasser, J., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, Faculty of Biology, University of Freiburg, Freiburg, 79104, Germany; Funk, K., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, Faculty of Biology, University of Freiburg, Freiburg, 79104, Germany; Jahreis, G., Department of Biochemistry/Biotechnology, Martin Luther University Halle-Wittenberg, Halle, 06120, Germany; Pérez-Payá, E., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain, Instituto de Biomedicina de Valencia, Instituto de Biomedicina de Valencia-Consejo Superior de Investigaciones Científicas, Valencia, 46010, Spain; Mingarro, I., Departament de Bioquímica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina, Universitat de València, Burjassot, 46100, Spain; Edlich, F., Institute for Biochemistry and Molecular Biology, University of Freiburg, Freiburg, 79104, Germany, BIOSS, Centre for Biological Signaling Studies, University of Freiburg, Freiburg, 79104, Germany; Orzáez, M., Laboratory of Peptide and Protein Chemistry, Centro de Investigación Príncipe Felipe, Valencia, E-46012, Spain

Recommended Citation:
Andreu-Fernández V.,Sancho M.,Genovés A.,et al. Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(2)
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