globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1920779117
论文题名:
Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor
作者: Cleves P.A.; Tinoco A.I.; Bradford J.; Perrin D.; Bay L.K.; Pringle J.R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:46
起始页码: 28899
结束页码: 28905
语种: 英语
英文关键词: Acropora | genome editing | heat stress
Scopus关键词: CRISPR associated endonuclease Cas9 ; guide RNA ; heat shock transcription factor 1 ; ribonucleoprotein ; Acropora millepora ; animal experiment ; animal tissue ; Article ; clustered regularly interspaced short palindromic repeat ; controlled study ; CRISPR-CAS9 system ; embryo ; gene dosage ; gene editing ; gene function ; gene induction ; gene mutation ; heat stress ; heat tolerance ; HSF1 gene ; larva ; microinjection ; mortality ; mutagenesis ; mutation rate ; nonhuman ; phenotype ; priority journal ; sea anemone ; survival ; zygote
英文摘要: Reef-building corals are keystone species that are threatened by anthropogenic stresses including climate change. To investigate corals’ responses to stress and other aspects of their biology, numerous genomic and transcriptomic studies have been performed, generating many hypotheses about the roles of particular genes and molecular pathways. However, it has not generally been possible to test these hypotheses rigorously because of the lack of genetic tools for corals or closely related cnidarians. CRISPR technology seems likely to alleviate this problem. Indeed, we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotein complexes into fertilized eggs of the coral Acropora millepora can produce a sufficiently high frequency of mutations to detect a clear phenotype in the injected generation. Based in part on experiments in a sea-anemone model system, we targeted the gene encoding Heat Shock Transcription Factor 1 (HSF1) and obtained larvae in which >90% of the gene copies were mutant. The mutant larvae survived well at 27 °C but died rapidly at 34 °C, a temperature that did not produce detectable mortality over the duration of the experiment in wild-type (WT) larvae or larvae injected with Cas9 alone. We conclude that HSF1 function (presumably its induction of genes in response to heat stress) plays an important protective role in corals. More broadly, we conclude that CRISPR mutagenesis in corals should allow wide-ranging and rigorous tests of gene function in both larval and adult corals. © 2020 National Academy of Sciences. All rights reserved.
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被引频次[WOS]:48   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163944
Appears in Collections:气候变化与战略

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作者单位: Cleves, P.A., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Tinoco, A.I., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Bradford, J., Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD 4001, Australia; Perrin, D., Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD 4001, Australia; Bay, L.K., Australian Institute of Marine Science, Townsville, QLD 4810, Australia; Pringle, J.R., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States

Recommended Citation:
Cleves P.A.,Tinoco A.I.,Bradford J.,et al. Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(46)
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