DOI: 10.1073/pnas.2015737117
论文题名: Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system
作者: Cleves P.A. ; Krediet C.J. ; Lehnert E.M. ; Onishi M. ; Pringle J.R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 46 起始页码: 28906
结束页码: 28917
语种: 英语
英文关键词: Heat-shock proteins | innate immunity | symbiosis | nutrient transport | reactive oxygen species
Scopus关键词: heat shock transcription factor 1
; immunoglobulin enhancer binding protein
; animal husbandry
; apoptosis
; Article
; bleaching
; controlled study
; coral
; down regulation
; gene expression
; gene function
; gene identification
; gene targeting
; genetic analysis
; genetic transcription
; heat stress
; immune response
; innate immunity
; nonhuman
; oxidative stress
; priority journal
; protein folding
; protein homeostasis
; RNA isolation
; RNA sequencing
; sea anemone
; symbiosis
; thermal exposure
; upregulation
英文摘要: Loss of endosymbiotic algae (“bleaching”) under heat stress has become a major problem for reef-building corals worldwide. To identify genes that might be involved in triggering or executing bleaching, or in protecting corals from it, we used RNAseq to analyze gene-expression changes during heat stress in a coral relative, the sea anemone Aiptasia. We identified >500 genes that showed rapid and extensive up-regulation upon temperature increase. These genes fell into two clusters. In both clusters, most genes showed similar expression patterns in symbiotic and aposymbiotic anemones, suggesting that this early stress response is largely independent of the symbiosis. Cluster I was highly enriched for genes involved in innate immunity and apoptosis, and most transcript levels returned to baseline many hours before bleaching was first detected, raising doubts about their possible roles in this process. Cluster II was highly enriched for genes involved in protein folding, and most transcript levels returned more slowly to baseline, so that roles in either promoting or preventing bleaching seem plausible. Many of the genes in clusters I and II appear to be targets of the transcription factors NFκB and HSF1, respectively. We also examined the behavior of 337 genes whose much higher levels of expression in symbiotic than aposymbiotic anemones in the absence of stress suggest that they are important for the symbiosis. Unexpectedly, in many cases, these expression levels declined precipitously long before bleaching itself was evident, suggesting that loss of expression of symbiosis-supporting genes may be involved in triggering bleaching. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163945
Appears in Collections: 气候变化与战略
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作者单位: Cleves, P.A., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Krediet, C.J., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States, Department of Marine Science, Eckerd College, St. Petersburg, FL 33711, United States; Lehnert, E.M., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Onishi, M., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States; Pringle, J.R., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States
Recommended Citation:
Cleves P.A.,Krediet C.J.,Lehnert E.M.,et al. Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(46)