globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2006534117
论文题名:
Developing a Population-state Decision System for Intelligently Reprogramming Extracellular Electron Transfer in Shewanella Oneidensis
作者: Li F.-H.; Tang Q.; Fan Y.-Y.; Li Y.; Li J.; Wu J.-H.; Luo C.-F.; Sun H.; Li W.-W.; Yu H.-Q.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:37
起始页码: 23001
结束页码: 23010
语种: 英语
英文关键词: Cr (VI) reduction ; Extracellular electron transfer (EET) ; Intelligently reprogramming ; Population-state decision ; Quorum sensing
Scopus关键词: chromium ; methyl orange ; chromium hexavalent ion ; Article ; bacterial gene ; bacterial genome ; bacterial growth ; bacterial strain ; biomass ; controlled study ; electron transport ; gene expression ; gene structure ; metabolic flux analysis ; nonhuman ; priority journal ; promoter region ; quorum sensing ; Shewanella oneidensis ; cell respiration ; electron ; electron transport ; metabolism ; physiology ; Shewanella ; Cell Respiration ; Chromium ; Electron Transport ; Electrons ; Quorum Sensing ; Shewanella
英文摘要: The unique extracellular electron transfer (EET) ability has positioned electroactive bacteria (EAB) as a major class of cellular chassis for genetic engineering aimed at favorable environmental, energy, and geoscience applications. However, previous efforts to genetically enhance EET ability have often impaired the basal metabolism and cellular growth due to the competition for the limited cellular resource. Here, we design a quorum sensing-based population-state decision (PSD) system for intelligently reprogramming the EET regulation system, which allows the rebalanced allocation of the cellular resource upon the bacterial growth state. We demonstrate that the electron output from Shewanella oneidensis MR-1 could be greatly enhanced by the PSD system via shifting the dominant metabolic flux from initial bacterial growth to subsequent EET enhancement (i.e., after reaching a certain population-state threshold). The strain engineered with this system achieved up to 4.8-fold EET enhancement and exhibited a substantially improved pollutant reduction ability, increasing the reduction efficiencies of methyl orange and hexavalent chromium by 18.8- and 5.5-fold, respectively. Moreover, the PSD system out-competed the constant expression system in managing EET enhancement, resulting in considerably enhanced electron output and pollutant bioreduction capability. The PSD system provides a powerful tool for intelligently managing extracellular electron transfer and may inspire the development of new-generation smart bioelectrical devices for various applications. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/164042
Appears in Collections:气候变化与战略

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作者单位: Li, F.-H., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China; Tang, Q., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China; Fan, Y.-Y., School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China; Li, Y., School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China; Li, J., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China; Wu, J.-H., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China; Luo, C.-F., School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China; Sun, H., School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China; Li, W.-W., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China; Yu, H.-Q., Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, University of Science and Technology of China, Hefei, 230026, China, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China

Recommended Citation:
Li F.-H.,Tang Q.,Fan Y.-Y.,et al. Developing a Population-state Decision System for Intelligently Reprogramming Extracellular Electron Transfer in Shewanella Oneidensis[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(37)
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