globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2002759117
论文题名:
Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition
作者: Gao C.; Xu H.; Huang J.; Sun B.; Zhang F.; Savage Z.; Duggan C.; Yan T.; Wu C.-H.; Wang Y.; Vleeshouwers V.G.A.A.; Kamoun S.; Bozkurt T.O.; Dong S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:17
起始页码: 9613
结束页码: 9620
语种: 英语
英文关键词: Chloroplast ; Disease resistance ; Light ; NLR ; Potato blight
Scopus关键词: glycerate 3 kinase ; phosphotransferase ; protein ; protein AVRvnt1 ; unclassified drug ; fungal protein ; glycerate kinase ; nucleotide binding oligomerization domain like receptor ; phosphotransferase ; plant protein ; Agrobacterium ; antigen recognition ; Article ; chloroplast ; confocal microscopy ; controlled study ; disease resistance ; energy yield ; gene silencing ; immune response ; immunoprecipitation ; in vivo study ; light ; nonhuman ; Phytophthora infestans ; plant immunity ; plasmid ; priority journal ; promoter region ; protein binding ; protein secretion ; reverse transcription polymerase chain reaction ; separation technique ; Western blotting ; animal ; chloroplast ; Escherichia coli ; gene expression regulation ; genetics ; immunology ; light ; metabolism ; microbiology ; Phytophthora infestans ; potato ; radiation response ; seedling ; tobacco ; two hybrid system ; Agrobacterium ; Animals ; Chloroplasts ; Escherichia coli ; Fungal Proteins ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Gene Silencing ; Light ; Microscopy, Confocal ; NLR Proteins ; Phosphotransferases (Alcohol Group Acceptor) ; Phytophthora infestans ; Plant Proteins ; Seedlings ; Solanum tuberosum ; Tobacco ; Two-Hybrid System Techniques
英文摘要: In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune sensors that recognize and eliminate a wide range of invading pathogens. NLR-mediated immunity is known to be modulated by environmental factors. However, how pathogen recognition by NLRs is influenced by environmental factors such as light remains unclear. Here, we show that the agronomically important NLR Rpi-vnt1.1 requires light to confer disease resistance against races of the Irish potato famine pathogen Phytophthora infestans that secrete the effector protein AVRvnt1. The activation of Rpi-vnt1.1 requires a nuclear-encoded chloroplast protein, glycerate 3-kinase (GLYK), implicated in energy production. The pathogen effector AVRvnt1 binds the full-length chloroplast-targeted GLYK isoform leading to activation of Rpi-vnt1.1. In the dark, Rpi-vnt1.1-mediated resistance is compromised because plants produce a shorter GLYK-lacking the intact chloroplast transit peptide-that is not bound by AVRvnt1. The transition between full-length and shorter plant GLYK transcripts is controlled by a light-dependent alternative promoter selection mechanism. In plants that lack Rpi-vnt1.1, the presence of AVRvnt1 reduces GLYK accumulation in chloroplasts counteracting GLYK contribution to basal immunity. Our findings revealed that pathogen manipulation of chloroplast functions has resulted in a light-dependent immune response. © 2020 National Academy of Sciences. All rights reserved.
Citation statistics:
被引频次[WOS]:30   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/164226
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Gao, C., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Xu, H., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Huang, J., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Sun, B., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Zhang, F., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Savage, Z., Imperial College, South Kensington Campus, London, SW7 2AZ, United Kingdom; Duggan, C., Imperial College, South Kensington Campus, London, SW7 2AZ, United Kingdom; Yan, T., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Wu, C.-H., Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UH, United Kingdom; Wang, Y., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China, Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, 210095, China; Vleeshouwers, V.G.A.A., Wageningen University and Research Plant Breeding, Wageningen University and Research, Wageningen, 6708 PB, Netherlands; Kamoun, S., Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UH, United Kingdom; Bozkurt, T.O., Imperial College, South Kensington Campus, London, SW7 2AZ, United Kingdom; Dong, S., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China, Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UH, United Kingdom, Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, 210095, China

Recommended Citation:
Gao C.,Xu H.,Huang J.,et al. Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(17)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao C.]'s Articles
[Xu H.]'s Articles
[Huang J.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Gao C.]'s Articles
[Xu H.]'s Articles
[Huang J.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao C.]‘s Articles
[Xu H.]‘s Articles
[Huang J.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.