globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1922714117
论文题名:
A paradigm of thermal adaptation in penguins and elephants by tuning cold activation in TRPM8
作者: Yang S.; Lu X.; Wang Y.; Xu L.; Chen X.; Yang F.; Lai R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:15
起始页码: 8633
结束页码: 8638
语种: 英语
英文关键词: Cold activation ; Pore domain ; Side-chain hydrophobicity ; Thermal adaptation ; TRPM8
Scopus关键词: transient receptor potential channel M8 ; transient receptor potential channel M ; animal experiment ; Article ; cold acclimatization ; cold tolerance ; conformational transition ; elephant ; environmental temperature ; hydrophobicity ; molecular evolution ; molecular imaging ; nonhuman ; patch clamp technique ; penguin ; priority journal ; protein analysis ; protein function ; structural model ; thermodynamics ; adaptation ; amino acid sequence ; animal ; channel gating ; cold ; elephant ; genetics ; metabolism ; penguin ; physiology ; sequence homology ; Adaptation, Physiological ; Amino Acid Sequence ; Animals ; Cold Temperature ; Elephants ; Ion Channel Gating ; Sequence Homology ; Spheniscidae ; TRPM Cation Channels
英文摘要: To adapt to habitat temperature, vertebrates have developed sophisticated physiological and ecological mechanisms through evolution. Transient receptor potential melastatin 8 (TRPM8) serves as the primary sensor for cold. However, how cold activates TRPM8 and how this sensor is tuned for thermal adaptation remain largely unknown. Here we established a molecular framework of how cold is sensed in TRPM8 with a combination of patch-clamp recording, unnatural amino acid imaging, and structural modeling. We first observed that the maximum cold activation of TRPM8 in eight different vertebrates (i.e., African elephant and emperor penguin) with distinct side-chain hydrophobicity (SCH) in the pore domain (PD) is tuned to match their habitat temperature. We further showed that altering SCH for residues in the PD with solvent-accessibility changes leads to specific tuning of the cold response in TRPM8. We also observed that knockin mice expressing the penguin's TRPM8 exhibited remarkable tolerance to cold. Together, our findings suggest a paradigm of thermal adaptation in vertebrates, where the evolutionary tuning of the cold activation in the TRPM8 ion channel through altering SCH and solvent accessibility in its PD largely contributes to the setting of the cold-sensitive/ tolerant phenotype. © 2020 National Academy of Sciences. All rights reserved.
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被引频次[WOS]:35   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/164250
Appears in Collections:气候变化与战略

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作者单位: Yang, S., Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China; Lu, X., Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, College of Life Sciences, University of Chinese Academy of Sciences, Bejing, 100049, China; Wang, Y., Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China; Xu, L., Department of Biophysics and Kidney Disease Center, First Affiliated Hospital, Institute of Neuroscience, National Health Commission, Chinese Academy of Medical Sciences, Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China; Chen, X., Department of Biophysics and Kidney Disease Center, First Affiliated Hospital, Institute of Neuroscience, National Health Commission, Chinese Academy of Medical Sciences, Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China; Yang, F., Department of Biophysics and Kidney Disease Center, First Affiliated Hospital, Institute of Neuroscience, National Health Commission, Chinese Academy of Medical Sciences, Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China; Lai, R., Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, Kunming Institute of Zoology, Chinese University of Hong-Kong, Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, Sino-African Joint Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China, Institute for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, 201203, China

Recommended Citation:
Yang S.,Lu X.,Wang Y.,et al. A paradigm of thermal adaptation in penguins and elephants by tuning cold activation in TRPM8[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(15)
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