globalchange  > 气候变化与战略
DOI: 10.1016/j.earscirev.2019.102887
论文题名:
Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation
作者: Armbrecht L.H.; Coolen M.J.L.; Lejzerowicz F.; George S.C.; Negandhi K.; Suzuki Y.; Young J.; Foster N.R.; Armand L.K.; Cooper A.; Ostrowski M.; Focardi A.; Stat M.; Moreau J.W.; Weyrich L.S.
刊名: Earth Science Reviews
ISSN: 00128252
出版年: 2019
卷: 196
语种: 英语
中文关键词: Ancient DNA ; Biomarkers ; Contamination ; Deep biosphere ; IODP ; Marine sediments ; Mars ; Phytoplankton ; Seafloor
英文关键词: biomarker ; biosphere ; DNA ; marine sediment ; Mars ; paleoecology ; phytoplankton ; pollutant ; seafloor ; Eukaryota ; Prokaryota
英文摘要: The study of ancient DNA (aDNA) from sediments (sedaDNA) offers great potential for paleoclimate interpretation, and has recently been applied as a tool to characterise past marine life and environments from deep ocean sediments over geological timescales. Using sedaDNA, palaeo-communities have been detected, including prokaryotes and eukaryotes that do not fossilise, thereby revolutionising the scope of marine micropalaeontological research. However, many studies to date have not reported on the measures taken to prove the authenticity of sedaDNA-derived data from which conclusions are drawn. aDNA is highly fragmented and degraded and extremely sensitive to contamination by non-target environmental DNA. Contamination risks are particularly high on research vessels, drilling ships and platforms, where logistics and facilities do not yet allow for sterile sediment coring, and due consideration needs to be given to sample processing and analysis following aDNA guidelines. This review clarifies the use of aDNA terminology, discusses common pitfalls and highlights the urgency behind adopting new standards for marine sedaDNA research, with a focus on sampling optimisation to facilitate the incorporation of routine sedaDNA research into International Ocean Discovery Program (IODP) operations. Currently available installations aboard drilling ships and platforms are reviewed, improvements suggested, analytical approaches detailed, and the controls and documentation necessary to support the authenticity of aDNA retrieved from deep-sea sediment cores is outlined. Beyond practical considerations, concepts relevant to the study of past marine biodiversity based on sedaDNA, and the applicability of the new guidelines to the study of other contamination-susceptible environments (permafrost and outer space) are discussed. © 2019 Elsevier B.V.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/165763
Appears in Collections:气候变化与战略

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作者单位: Australian Centre for Ancient DNA, School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Australia; Western Australian Organic and Isotope Geochemistry Centre (WA-OIGC), School of Earth and Planetary Sciences, Curtin University, Kent Street, Bentley, WA 6102, Australia; Center for Microbiome Innovation, University of California, San Diego, La Jolla, CA 92093-0403, United States; Department of Earth and Planetary Sciences and MQMarine Research Centre, Macquarie University, North Ryde, NSW 2109, Australia; NSW Office of Environment and Heritage, 10 Valentine Avenue, Parramatta, NSW 2150, Australia; Department of Earth and Planetary Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan; Advanced DNA, Identification and Forensic Facility, The Environment Institute, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia; School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Australia; Australian and New Zealand IODP Consortium, Research School of Earth Sciences, The Australian National University, Acton, ACT 2601, Australia; Centre of Excellence for Australian Biodiversity and Heritage, School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Australia; Climate Change Cluster, Faculty of Science, University of Technology Sydney, Chippendale, NSW 2007, Australia; Department of Molecular Sciences and MQMarine Research Centre, Macquarie University, North Ryde, NSW 2109, Australia; School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia; School of Earth Sciences, The University of Melbourne, Parkville, VIC 3010, Australia; School of Geographical & Earth Sciences, University of Glasgow, Lilybank Gardens, Glasgow, G12 8QQ, United Kingdom

Recommended Citation:
Armbrecht L.H.,Coolen M.J.L.,Lejzerowicz F.,et al. Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation[J]. Earth Science Reviews,2019-01-01,196
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