globalchange  > 气候变化与战略
DOI: 10.1016/j.scib.2021.07.003
论文题名:
Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum
作者: Dong C.; Qu G.; Guo J.; Wei F.; Gao S.; Sun Z.; Jin L.; Sun X.; Rochaix J.-D.; Miao Y.; Wang R.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
语种: 英语
中文关键词: Carotenoid ; Geranylgeranyl diphosphate synthase (GGPPS) ; Methylerythritol phosphate pathway (MEP) ; Nicotiana tabacum ; Rational design
英文摘要: Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and transgenesis. In this study, we demonstrate that rational design enables the engineering of geranylgeranyl diphosphate synthase (NtGGPPS), an enzyme of the methylerythritol phosphate pathway (MEP) in the model plant Nicotiana tabacum. As the crucial bottleneck in carotenoid biosynthesis, NtGGPPS1 interacts with phytoene synthase (NtPSY1) to channel GGPP into the production of carotenoids. Loss of this enzyme in the ntggpps1 mutant leads to decreased carotenoid accumulation. With the aim of enhancing NtGGPPS1 activity, we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence alignment. The activity of the designed NtGGPPS1 (a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E, d-NtGGPPS1) was measured by a high-throughput colorimetric assay. d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate (DMAPP ~1995.5-fold, GPP ~25.9-fold, and FPP ~16.7-fold) for GGPP synthesis compared with wild-type NtGGPPS1. Importantly, the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves, improved photosynthetic efficiency, and increased biomass relative to NtGGPPS1. These findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield traits. Our results open the door for the structure-guided rational design of elite genes in higher plants. © 2021 Science China Press
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170234
Appears in Collections:气候变化与战略

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作者单位: Zhengzhou Tobacco Research Institute, Zhengzhou, 450001, China; Key Laboratory of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng, 475001, China; College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China; Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, 1211, Switzerland

Recommended Citation:
Dong C.,Qu G.,Guo J.,et al. Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum[J]. Science Bulletin,2021-01-01
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