globalchange  > 气候变化与战略
DOI: 10.1016/j.scib.2021.06.019
论文题名:
Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila
作者: Gao Y.; Liu S.; Jia Q.; Wu L.; Yuan D.; Li E.Y.; Feng Q.; Wang G.; Palli S.R.; Wang J.; Li S.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
语种: 英语
中文关键词: 20-Hydroxyecdysone action ; Juvenile hormone ; Phosphoproteomics ; Receptor tyrosine kinase ; USP ; Yorkie activity
英文关键词: Biochemistry ; Cell signaling ; Complex networks ; Enzymes ; Gene expression ; Hormones ; Phosphorylation ; Signaling ; 20-hydroxyecdysone action ; Drosophilla ; Intracellular receptors ; Juvenile hormone ; Methoprene ; Phosphoproteomics ; Protein kinases c ; Receptor-tyrosine kinase ; Ultraspiracle ; Yorkie activity ; Amino acids
英文摘要: Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant (Met) and germ-cell expressed (Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. The uspS35A mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20E signaling that delayed developmental timing. The uspS35A mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development. This study helps better understand the complex JH signaling network. © 2021 Science China Press
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被引频次[WOS]:12   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170251
Appears in Collections:气候变化与战略

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作者单位: Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, 514779, China; International Department, The Affiliated High School of South China Normal University, Guangzhou, 510631, China; Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, 40546, United States; Department of Entomology, University of Maryland, College Park, 20742, United States

Recommended Citation:
Gao Y.,Liu S.,Jia Q.,et al. Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila[J]. Science Bulletin,2021-01-01
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