DOI: 10.1016/j.scib.2021.02.003
论文题名: m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish
作者: Ren F. ; Miao R. ; Xiao R. ; Mei J.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
卷: 66, 期: 11 起始页码: 1119
结束页码: 1128
语种: 英语
中文关键词: Bucky ball
; Germ cell specification
; Germ plasm assembly
; Igf2bp3
; m6A reader
; m6A-dependent regulation
英文关键词: Gene expression
; Bucky ball
; Genes expression
; Germ cell specification
; Germ plasm assembly
; Igf2bp3
; M6A reader
; M6A-dependent regulation
; mRNA expression
; Zebrafish
; Zebrafish development
; Specifications
英文摘要: Bucky ball (Buc) is involved in germ plasm (GP) assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism. The present study demonstrates that an m6A reader Igf2bp3 interacts and colocalizes with Buc in the GP. Similar to the loss of Buc, the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification, which can be partially restored by the injection of igf2bp3 mRNA. Igf2bp3 binds to m6A-modified GP-organizer and GP mRNAs in an m6A-dependent manner and prevents their degradation. These findings indicate that the functions of Igf2bp3, a direct effector protein of Buc, in GP mRNA expression and GP assembly involve m6A-dependent regulation; these results emphasize a critical role of m6A modification in the process of GP assembly. © 2021 Science China Press Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170377
Appears in Collections: 气候变化与战略
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作者单位: College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, 430071, China; Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430071, China
Recommended Citation:
Ren F.,Miao R.,Xiao R.,et al. m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish[J]. Science Bulletin,2021-01-01,66(11)