DOI: 10.1016/j.scib.2020.11.003
论文题名: Unanchored ubiquitin chain sustains RIG-I-induced interferon-I activation and controls selective gene expression
作者: Xian H. ; Huang W. ; Sun T. ; Yang S. ; Zhang C. ; Wang J. ; Zhang Y. ; Cui J.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
卷: 66, 期: 8 起始页码: 794
结束页码: 802
语种: 英语
中文关键词: Antiviral immunity
; Cellular homeostasis
; RIG-I
; Type I interferon
; Ubiquitination
; Unanchored ubiquitin chains
英文关键词: Antibiotics
; Chemical activation
; Gene expression
; Glycoproteins
; Antiviral response
; Binding abilities
; Cell fate decision
; Induced interferons
; Mathematical analysis
; Regulatory mechanism
; Sustained activation
; Type i interferons
; Activation analysis
英文摘要: Ubiquitination plays a crucial role in retinoic acid-inducible gene I (RIG-I)-induced antiviral responses. However, the precise regulatory mechanisms of RIG-I activity mediated by conjugated and unanchored ubiquitin chains remain to be determined. In this study, we discovered that T55 of RIG-I was required for its binding ability for the unanchored ubiquitin chains. Experimental and mathematical analysis showed that unanchored ubiquitin chains associated with RIG-I were essential for sustained activation of type I interferon (IFN) signaling. Transcriptomics study revealed that the binding of RIG-I with unanchored ubiquitin chains additionally regulated the expression of a subset of metabolic and cell fate decision genes. Moreover, we found that ubiquitin-specific peptidase 21 (USP21) and USP3 deubiquitinate conjugated and unanchored ubiquitin chains on RIG-I respectively. Taken together, characterization of the regulation mode and functions of conjugated ubiquitination and the unconjugated ubiquitin chain-binding of RIG-I may provide means to fine-tune RIG-I-mediated type I IFN signaling. © 2020 Science China Press Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170450
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Department of Gastroenterology, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China; School of Life Sciences, Anqing Normal University, Anqing, 246133, China
Recommended Citation:
Xian H.,Huang W.,Sun T.,et al. Unanchored ubiquitin chain sustains RIG-I-induced interferon-I activation and controls selective gene expression[J]. Science Bulletin,2021-01-01,66(8)