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DOI: 10.1371/journal.pone.0101924
论文题名:
Rapid MALDI-TOF Mass Spectrometry Strain Typing during a Large Outbreak of Shiga-Toxigenic Escherichia coli
作者: Martin Christner; Maria Trusch; Holger Rohde; Marcel Kwiatkowski; Hartmut Schlüter; Manuel Wolters; Martin Aepfelbacher; Moritz Hentschke
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-7-8
卷: 9, 期:7
语种: 英语
英文关键词: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry ; Biomarkers ; Formic acid ; Acid deposition ; Signal to noise ratio ; Polymerase chain reaction ; Protein sequencing ; Sequence databases
英文摘要: Background In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification. Methods Specific peaks in the outbreak strain’s spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak. Results Comparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates. Conclusions MALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101924&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/19082
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Institute of Organic Chemistry, Mass Spectrometry Facility, University of Hamburg, Hamburg, Germany;Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Clinical Chemistry, Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Clinical Chemistry, Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

Recommended Citation:
Martin Christner,Maria Trusch,Holger Rohde,et al. Rapid MALDI-TOF Mass Spectrometry Strain Typing during a Large Outbreak of Shiga-Toxigenic Escherichia coli[J]. PLOS ONE,2014-01-01,9(7)
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