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DOI: 10.1371/journal.pone.0098739
论文题名:
Streptococcus pneumoniae Carriage Prevalence in Nepal: Evaluation of a Method for Delayed Transport of Samples from Remote Regions and Implications for Vaccine Implementation
作者: Sarah Hanieh; Mainga Hamaluba; Dominic F. Kelly; Jane A. Metz; Kelly L. Wyres; Roberta Fisher; Rahul Pradhan; Disuja Shakya; Lochan Shrestha; Amrita Shrestha; Anip Joshi; Jocelyn Habens; Bishnu D. Maharjan; Stephen Thorson; Erik Bohler; Ly-Mee Yu; Sarah Kelly; Emma Plested; Tessa John; Anja M. Werno; Neelam Adhikari; David R. Murdoch; Angela B. Brueggemann; Andrew J. Pollard
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-6-6
卷: 9, 期:6
语种: 英语
英文关键词: Conjugate vaccines ; Nepal ; Vaccines ; Children ; Phylogeography ; Pneumococcus ; Polymerase chain reaction ; Genotyping
英文摘要: Background Pneumococcal disease is a significant cause of morbidity and mortality in young children in Nepal, and currently available pneumococcal conjugate vaccines offer moderate coverage of invasive disease isolates. Methods A prevalence study of children aged 1.5 to 24 months in urban and rural Nepal was conducted. In the urban group, nasopharyngeal swabs (NPS) were transported using silica desiccant packages (SDP) with delayed processing (2 weeks), or skim-milk-tryptone-glucose-glycerin (STGG) with immediate processing (within 8 hours). Pneumococcal nasopharyngeal carriage prevalence, serogroup/type distribution and isolate genotypes (as defined by multilocus sequence typing) were determined. Results 1101 children were enrolled into the study: 574 in the urban group and 527 in the rural group. Overall carriage prevalence based on culture from specimens transported and stored in STGG was 58.7% (337/574), compared to 40.9% (235/574) in SDP. There was concordance of detection of pneumococcus in 67% of samples. Using the SDP method, pneumococcal carriage prevalence was higher in the rural population (69.2%; 364/526) compared to the urban population (40.9%; 235/574). Serogroup/type distribution varied with geographical location. Over half of the genotypes identified in both the urban and rural pneumococcal populations were novel. Conclusion The combination of delayed culture and transport using SDP underestimates the prevalence of pneumococcal carriage; however, in remote areas, this method could still provide a useful estimate of carriage prevalence and serogroup/type distribution. Vaccine impact is unpredictable in a setting with novel genotypes and limited serotype coverage as described here. Consequently, continued surveillance of pneumococcal isolates from carriage and disease in Nepali children following the planned introduction of pneumococcal conjugate vaccines introduction will be essential.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0098739&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/19101
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Department of Zoology, University of Oxford, Oxford, United Kingdom;Department of Zoology, University of Oxford, Oxford, United Kingdom;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Okhaldhunga Community Hospital, Okhaldhunga, Nepal;Nuffield Department of Primary Care and Health Sciences, University of Oxford, Oxford, United Kingdom;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom;Department of Pathology, University of Otago, Christchurch, New Zealand;Canterbury Health Laboratories, Christchurch, New Zealand;Patan Academy Paediatric Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal;Department of Pathology, University of Otago, Christchurch, New Zealand;Canterbury Health Laboratories, Christchurch, New Zealand;Department of Zoology, University of Oxford, Oxford, United Kingdom;Oxford Vaccine Group, Department of Paediatrics, University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom

Recommended Citation:
Sarah Hanieh,Mainga Hamaluba,Dominic F. Kelly,et al. Streptococcus pneumoniae Carriage Prevalence in Nepal: Evaluation of a Method for Delayed Transport of Samples from Remote Regions and Implications for Vaccine Implementation[J]. PLOS ONE,2014-01-01,9(6)
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