Salt stress is a major abiotic stress that limits crop productivity in many regions of the world. A comparative proteomic approach to identify salt stress-responsive proteins and to understand the molecular mechanisms was carried out in the woody halophyte Kandelia candel. Four-leaf-old K. candel seedlings were exposed to 150 (control), 300, 450, and 600 mM NaCl for 3 days. Proteins extracted from the leaves of K. candel seedlings were separated by two-dimensional gel electrophoresis (2-DE). More than 900 protein spots were detected on each gel, and 53 differentially expressed protein spots were located with at least two-fold differences in abundance on 2-DE maps, of which 48 were identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF-TOF/MS). The results showed that K. candel could withstand up to 450 mM NaCl stress by up-regulating proteins that are mainly involved in photosynthesis, respiration and energy metabolism, Na+ compartmentalization, protein folding and assembly, and signal transduction. Physiological data, including superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities, hydrogen peroxide (H2O2) and superoxide anion radicals (O2−) contents, as well as Na+ content and K+/Na+ ratios all correlated well with our proteomic results. This study provides new global insights into woody halophyte salt stress responses. Identification of differentially expressed proteins promotes better understanding of the molecular basis for salt stress reduction in K. candel.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Fujian Academy of Forestry, Fuzhou, Fujian, China;School of Life Sciences, Ningxia University, Yinchuan, Ningxia, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Fujian Academy of Forestry, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
Recommended Citation:
Lingxia Wang,Xiao Liu,Meng Liang,et al. Proteomic Analysis of Salt-Responsive Proteins in the Leaves of Mangrove Kandelia candel during Short-Term Stress[J]. PLOS ONE,2014-01-01,9(1)