Dengue Virus 2 American-Asian Genotype Identified during the 2006/2007 Outbreak in Piauí, Brazil Reveals a Caribbean Route of Introduction and Dissemination of Dengue Virus in Brazil
Dengue virus (DENV) is the most widespread arthropod-borne virus, and the number and severity of outbreaks has increased worldwide in recent decades. Dengue is caused by DENV-1, DENV- 2, DENV-3 and DENV-4 which are genetically distant. The species has been subdivided into genotypes based on phylogenetic studies. DENV-2, which was isolated from dengue fever patients during an outbreak in Piaui, Brazil in 2006/2007 was analyzed by sequencing the envelope (E) gene. The results indicated a high similarity among the isolated viruses, as well as to other DENV-2 from Brazil, Central America and South America. A phylogenetic and phylogeographic analysis based on DENV-2E gene sequences revealed that these viruses are grouped together with viruses of the American-Asian genotype in two distinct lineages. Our results demonstrate the co-circulation of two American-Asian genotype lineages in northeast Brazil. Moreover, we reveal that DENV-2 lineage 2 was detected in Piauí before it disseminated to other Brazilian states and South American countries, indicating the existence of a new dissemination route that has not been previously described.
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Laboratório de Biodados, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Laboratório de Vacinas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil;Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;Laboratório de Biotecnologia, Universidade Federal do Piauí, Parnaíba, Piauí, Brazil;Laboratório de Imunologia Celular e Molecular, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil;Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Recommended Citation:
Leandra Barcelos Figueiredo,Tetsu Sakamoto,Luiz Felipe Leomil Coelho,et al. Dengue Virus 2 American-Asian Genotype Identified during the 2006/2007 Outbreak in Piauí, Brazil Reveals a Caribbean Route of Introduction and Dissemination of Dengue Virus in Brazil[J]. PLOS ONE,2014-01-01,9(8)