gchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0106135
Title:
DNA Barcoding for Species Assignment: The Case of Mediterranean Marine Fishes
Author: Monica Landi; Mark Dimech; Marco Arculeo; Girolama Biondo; Rogelia Martins; Miguel Carneiro; Gary Robert Carvalho; Sabrina Lo Brutto; Filipe O. Costa
Source Publication: PLOS ONE
ISSN: 1932-6203
Indexed By: SCI-E
Publishing Year: 2014
Date Published: 2014-9-15
Volume: 9, Issue:9
Language: 英语
Keyword: DNA barcoding ; Marine fish ; DNA libraries ; Taxonomy ; Mediterranean Sea ; Sequence databases ; Marine biology ; Cryptic speciation
Subject in Chinese: 渔业 ; 保护
Subject: FISHERIES ; CONSERVATION
English Abstract: Background DNA barcoding enhances the prospects for species-level identifications globally using a standardized and authenticated DNA-based approach. Reference libraries comprising validated DNA barcodes (COI) constitute robust datasets for testing query sequences, providing considerable utility to identify marine fish and other organisms. Here we test the feasibility of using DNA barcoding to assign species to tissue samples from fish collected in the central Mediterranean Sea, a major contributor to the European marine ichthyofaunal diversity. Methodology/Principal Findings A dataset of 1278 DNA barcodes, representing 218 marine fish species, was used to test the utility of DNA barcodes to assign species from query sequences. We tested query sequences against 1) a reference library of ranked DNA barcodes from the neighbouring North East Atlantic, and 2) the public databases BOLD and GenBank. In the first case, a reference library comprising DNA barcodes with reliability grades for 146 fish species was used as diagnostic dataset to screen 486 query DNA sequences from fish specimens collected in the central basin of the Mediterranean Sea. Of all query sequences suitable for comparisons 98% were unambiguously confirmed through complete match with reference DNA barcodes. In the second case, it was possible to assign species to 83% (BOLD-IDS) and 72% (GenBank) of the sequences from the Mediterranean. Relatively high intraspecific genetic distances were found in 7 species (2.2%–18.74%), most of them of high commercial relevance, suggesting possible cryptic species. Conclusion/Significance We emphasize the discriminatory power of COI barcodes and their application to cases requiring species level resolution starting from query sequences. Results highlight the value of public reference libraries of reliability grade-annotated DNA barcodes, to identify species from different geographical origins. The ability to assign species with high precision from DNA samples of disparate quality and origin has major utility in several fields, from fisheries and conservation programs to control of fish products authenticity.
Related Link: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106135&type=printable
Citation statistics:
Document Type: 期刊论文
Identifier: http://119.78.100.177/globalchange/handle/2HF3EXSE/19295
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

Files in This Item:
File Name/ File Size Content Type Version Access License
journal.pone.0106135.PDF(370KB)期刊论文作者接受稿开放获取View 联系获取全文

Affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal;Malta Centre for Fisheries Science (MCFS), Fort San Lucjan Marsaxlokk, Malta;Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Palermo, Italy;Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Palermo, Italy;Modelling and Management Fishery Resources Division (DIV-RP), Instituto Português do Mar e da Atmosfera, Lisboa, Portugal;Modelling and Management Fishery Resources Division (DIV-RP), Instituto Português do Mar e da Atmosfera, Lisboa, Portugal;Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, Bangor, United Kingdom;Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Palermo, Italy;Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal

Recommended Citation:
Monica Landi,Mark Dimech,Marco Arculeo,et al. DNA Barcoding for Species Assignment: The Case of Mediterranean Marine Fishes[J]. PLOS ONE,2014-01-01,9(9)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Monica Landi]'s Articles
[Mark Dimech]'s Articles
[Marco Arculeo]'s Articles
百度学术
Similar articles in Baidu Scholar
[Monica Landi]'s Articles
[Mark Dimech]'s Articles
[Marco Arculeo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Monica Landi]‘s Articles
[Mark Dimech]‘s Articles
[Marco Arculeo]‘s Articles
Related Copyright Policies
Null
收藏/分享
文件名: journal.pone.0106135.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.