全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0109327
论文题名:	Quantitative and Functional Characterization of the Hyper-Conserved Protein of Prochlorococcus and Marine Synechococcus
作者:	Caroline E. Whidden; Katrina G. DeZeeuw; Jackie K. Zorz; Andrew P. Joy; David A. Barnett; Milo S. Johnson; Olga Zhaxybayeva; Amanda M. Cockshutt
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-10-31
卷:	9, 期:10
语种:	英语
英文关键词:	Synechococcus ; Cyanobacteria ; Ribosomal RNA ; Marine conservation ; Ribosomes ; Comparative genomics ; Protein extraction ; Fresh water
英文摘要:	A large fraction of any bacterial genome consists of hypothetical protein-coding open reading frames (ORFs). While most of these ORFs are present only in one or a few sequenced genomes, a few are conserved, often across large phylogenetic distances. Such conservation provides clues to likely uncharacterized cellular functions that need to be elucidated. Marine cyanobacteria from the Prochlorococcus/marine Synechococcus clade are dominant bacteria in oceanic waters and are significant contributors to global primary production. A Hyper Conserved Protein (PSHCP) of unknown function is 100% conserved at the amino acid level in genomes of Prochlorococcus/marine Synechococcus, but lacks homologs outside of this clade. In this study we investigated Prochlorococcus marinus strains MED4 and MIT 9313 and Synechococcus sp. strain WH 8102 for the transcription of the PSHCP gene using RT-Q-PCR, for the presence of the protein product through quantitative immunoblotting, and for the protein's binding partners in a pull down assay. Significant transcription of the gene was detected in all strains. The PSHCP protein content varied between 8±1 fmol and 26±9 fmol per ug total protein, depending on the strain. The 50 S ribosomal protein L2, the Photosystem I protein PsaD and the Ycf48-like protein were found associated with the PSHCP protein in all strains and not appreciably or at all in control experiments. We hypothesize that PSHCP is a protein associated with the ribosome, and is possibly involved in photosystem assembly.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0109327&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19432
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:	Download All

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0109327.PDF（313KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Department of Chemistry & Biochemistry, Mount Allison University, Sackville, NB, Canada;Department of Chemistry & Biochemistry, Mount Allison University, Sackville, NB, Canada;Department of Chemistry & Biochemistry, Mount Allison University, Sackville, NB, Canada;Atlantic Cancer Research Institute, Moncton, NB, Canada;Atlantic Cancer Research Institute, Moncton, NB, Canada;Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America;Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America;Department of Computer Science, Dartmouth College, Hanover, New Hampshire, United States of America;Department of Chemistry & Biochemistry, Mount Allison University, Sackville, NB, Canada

Recommended Citation:

Caroline E. Whidden,Katrina G. DeZeeuw,Jackie K. Zorz,et al. Quantitative and Functional Characterization of the Hyper-Conserved Protein of Prochlorococcus and Marine Synechococcus[J]. PLOS ONE,2014-01-01,9(10)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Caroline E. Whidden]'s Articles
	[Katrina G. DeZeeuw]'s Articles
	[Jackie K. Zorz]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Caroline E. Whidden]'s Articles
	[Katrina G. DeZeeuw]'s Articles
	[Jackie K. Zorz]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Caroline E. Whidden]‘s Articles
	[Katrina G. DeZeeuw]‘s Articles
	[Jackie K. Zorz]‘s Articles
Related Copyright Policies
Null
收藏/分享