全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0102826
论文题名:	Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments
作者:	Richard A. Hurt Jr; Michael S. Robeson II; Migun Shakya; James G. Moberly; Tatiana A. Vishnivetskaya; Baohua Gu; Dwayne A. Elias
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-7-17
卷:	9, 期:7
语种:	英语
英文关键词:	Sequence databases ; DNA extraction ; Adsorption ; Sediment ; Phosphates ; RNA extraction ; Iron oxides ; Specimen grinding
英文摘要:	Despite over three decades of progress, extraction of high molecular weight (HMW) DNA from high clay soils or iron oxide cemented clay has remained challenging. HMW DNA is desirable for next generation sequencing as it yields the most comprehensive coverage. Several DNA extraction procedures were compared from samples that exhibit strong nucleic acid adsorption. pH manipulation or use of alternative ion solutions offered no improvement in nucleic acid recovery. Lysis by liquid N2 grinding in concentrated guanidine followed by concentrated sodium phosphate extraction supported HMW DNA recovery from clays high in iron oxides. DNA recovered using 1 M sodium phosphate buffer (PB) as a competitive desorptive wash was 15.22±2.33 µg DNA/g clay, with most DNA consisting of >20 Kb fragments, compared to 2.46±0.25 µg DNA/g clay with the Powerlyzer system (MoBio). Increasing PB concentration in the lysis reagent coincided with increasing DNA fragment length during initial extraction. Rarefaction plots of 16S rRNA (V1–V3 region) pyrosequencing from A-horizon and clay soils showed an ∼80% and ∼400% larger accessed diversity compared to the Powerlyzer soil DNA system, respectively. The observed diversity from the Firmicutes showed the strongest increase with >3-fold more operational taxonomic units (OTU) recovered.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0102826&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19531
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:	Download All

File Name/ File Size	Content Type	Version	Access	License
10.1371journal.pone.0102826.PDF（2033KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America;Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America;Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America;Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America;Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America;Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America;Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America

Recommended Citation:

Richard A. Hurt Jr,Michael S. Robeson II,Migun Shakya,et al. Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments[J]. PLOS ONE,2014-01-01,9(7)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Richard A. Hurt Jr]'s Articles
	[Michael S. Robeson II]'s Articles
	[Migun Shakya]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Richard A. Hurt Jr]'s Articles
	[Michael S. Robeson II]'s Articles
	[Migun Shakya]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Richard A. Hurt Jr]‘s Articles
	[Michael S. Robeson II]‘s Articles
	[Migun Shakya]‘s Articles
Related Copyright Policies
Null
收藏/分享