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DOI:	10.1371/journal.pone.0099994
论文题名:	Metagenomic Analysis Reveals Presence of Treponema denticola in a Tissue Biopsy of the Iceman
作者:	Frank Maixner; Anton Thomma; Giovanna Cipollini; Stefanie Widder; Thomas Rattei; Albert Zink
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-6-18
卷:	9, 期:6
语种:	英语
英文关键词:	Ribosomal RNA ; Opportunistic pathogens ; Genomic databases ; Paleogenetics ; Polymerase chain reaction ; Ancient DNA ; Genome analysis ; Sequence alignment
英文摘要:	Ancient hominoid genome studies can be regarded by definition as metagenomic analyses since they represent a mixture of both hominoid and microbial sequences in an environment. Here, we report the molecular detection of the oral spirochete Treponema denticola in ancient human tissue biopsies of the Iceman, a 5,300-year-old Copper Age natural ice mummy. Initially, the metagenomic data of the Iceman’s genomic survey was screened for bacterial ribosomal RNA (rRNA) specific reads. Through ranking the reads by abundance a relatively high number of rRNA reads most similar to T. denticola was detected. Mapping of the metagenome sequences against the T. denticola genome revealed additional reads most similar to this opportunistic pathogen. The DNA damage pattern of specifically mapped reads suggests an ancient origin of these sequences. The haematogenous spread of bacteria of the oral microbiome often reported in the recent literature could already explain the presence of metagenomic reads specific for T. denticola in the Iceman’s bone biopsy. We extended, however, our survey to an Iceman gingival tissue sample and a mouth swab sample and could thereby detect T. denticola and Porphyrimonas gingivalis, another important member of the human commensal oral microflora. Taken together, this study clearly underlines the opportunity to detect disease-associated microorganisms when applying metagenomics- enabled approaches on datasets of ancient human remains.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0099994&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/20033
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
10.1371journal.pone.0099994.PDF（1708KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Institute for Mummies and the Iceman, EURAC Research, Bolzano, Italy;CUBE - Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria;Institute for Mummies and the Iceman, EURAC Research, Bolzano, Italy;CUBE - Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria;CUBE - Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria;CUBE - Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria

Recommended Citation:

Frank Maixner,Anton Thomma,Giovanna Cipollini,et al. Metagenomic Analysis Reveals Presence of Treponema denticola in a Tissue Biopsy of the Iceman[J]. PLOS ONE,2014-01-01,9(6)

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