gchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0130407
Title:
Molecular Identification of Atlantic Bluefin Tuna (Thunnus thynnus, Scombridae) Larvae and Development of a DNA Character-Based Identification Key for Mediterranean Scombrids
Author: Gregory Neils Puncher; Haritz Arrizabalaga; Francisco Alemany; Alessia Cariani; Isik K. Oray; F. Saadet Karakulak; Gualtiero Basilone; Angela Cuttitta; Salvatore Mazzola; Fausto Tinti
Source Publication: PLOS ONE
ISSN: 1932-6203
Indexed By: SCI-E
Publishing Year: 2015
Date Published: 2015-7-6
Volume: 10, Issue:7
Language: 英语
Keyword: Larvae ; Sequence databases ; Tuna ; Sequence alignment ; BLAST algorithm ; DNA sequence analysis ; Fisheries ; Mediterranean Sea
Subject in Chinese: 渔业 ; ; 生物量
Subject: FISHERIES ; LEAD ; BIOMASS
English Abstract: The Atlantic bluefin tuna, Thunnus thynnus, is a commercially important species that has been severely over-exploited in the recent past. Although the eastern Atlantic and Mediterranean stock is now showing signs of recovery, its current status remains very uncertain and as a consequence their recovery is dependent upon severe management informed by rigorous scientific research. Monitoring of early life history stages can inform decision makers about the health of the species based upon recruitment and survival rates. Misidentification of fish larvae and eggs can lead to inaccurate estimates of stock biomass and productivity which can trigger demands for increased quotas and unsound management conclusions. Herein we used a molecular approach employing mitochondrial and nuclear genes (CO1 and ITS1, respectively) to identify larvae (n = 188) collected from three spawning areas in the Mediterranean Sea by different institutions working with a regional fisheries management organization. Several techniques were used to analyze the genetic sequences (sequence alignments using search algorithms, neighbour joining trees, and a genetic character-based identification key) and an extensive comparison of the results is presented. During this process various inaccuracies in related publications and online databases were uncovered. Our results reveal important differences in the accuracy of the taxonomic identifications carried out by different ichthyoplanktologists following morphology-based methods. While less than half of larvae provided were bluefin tuna, other dominant taxa were bullet tuna (Auxis rochei), albacore (Thunnus alalunga) and little tunny (Euthynnus alletteratus). We advocate an expansion of expertise for a new generation of morphology-based taxonomists, increased dialogue between morphology-based and molecular taxonomists and increased scrutiny of public sequence databases.
Related Link: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0130407&type=printable
Citation statistics:
被引频次[WOS]:10   [查看WOS记录]     [查看WOS中相关记录]
Document Type: 期刊论文
Identifier: http://119.78.100.177/globalchange/handle/2HF3EXSE/21175
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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Affiliation: Dept. of Biological, Geological and Environmental Sciences / Laboratory of Genetics and Genomics of Marine Resources and Environment (GenoDREAM), University of Bologna, Ravenna, Italy;AZTI Tecnalia, Marine Research Division, Herrera Kaia, Pasaia, Gipuzkoa, Spain;Instituto Español de Oceanografía, Centro Oceanográfico de Baleares, Palma, Spain;Dept. of Biological, Geological and Environmental Sciences / Laboratory of Genetics and Genomics of Marine Resources and Environment (GenoDREAM), University of Bologna, Ravenna, Italy;Istanbul University, Faculty of Fisheries, Laleli, Istanbul, Turkey;Istanbul University, Faculty of Fisheries, Laleli, Istanbul, Turkey;Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Trapani, Italy;Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Trapani, Italy;Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Trapani, Italy;Dept. of Biological, Geological and Environmental Sciences / Laboratory of Genetics and Genomics of Marine Resources and Environment (GenoDREAM), University of Bologna, Ravenna, Italy

Recommended Citation:
Gregory Neils Puncher,Haritz Arrizabalaga,Francisco Alemany,et al. Molecular Identification of Atlantic Bluefin Tuna (Thunnus thynnus, Scombridae) Larvae and Development of a DNA Character-Based Identification Key for Mediterranean Scombrids[J]. PLOS ONE,2015-01-01,10(7)
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