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DOI: 10.1371/journal.pone.0123298
论文题名:
Rapid High Resolution Genotyping of Francisella tularensis by Whole Genome Sequence Comparison of Annotated Genes (“MLST+”)
作者: Markus H. Antwerpen; Karola Prior; Alexander Mellmann; Sebastian Höppner; Wolf D. Splettstoesser; Dag Harmsen
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-4-9
卷: 10, 期:4
语种: 英语
英文关键词: Francisella tularensis ; Genomic databases ; Phylogeography ; Genome analysis ; Bacterial pathogens ; Nucleotide sequencing ; Genomic libraries ; Genotyping
英文摘要: The zoonotic disease tularemia is caused by the bacterium Francisella tularensis. This pathogen is considered as a category A select agent with potential to be misused in bioterrorism. Molecular typing based on DNA-sequence like canSNP-typing or MLVA has become the accepted standard for this organism. Due to the organism’s highly clonal nature, the current typing methods have reached their limit of discrimination for classifying closely related subpopulations within the subspecies F. tularensis ssp. holarctica. We introduce a new gene-by-gene approach, MLST+, based on whole genome data of 15 sequenced F. tularensis ssp. holarctica strains and apply this approach to investigate an epidemic of lethal tularemia among non-human primates in two animal facilities in Germany. Due to the high resolution of MLST+ we are able to demonstrate that three independent clones of this highly infectious pathogen were responsible for these spatially and temporally restricted outbreaks.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0123298&type=printable
Citation statistics:
被引频次[WOS]:35   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/21700
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Bundeswehr, Institute of Microbiology, Neuherbergstrasse 12, 80937, Muenchen, Germany;University of Muenster, Department of Periodontology, Waldeyerstrasse 30, 48149, Muenster, Germany;University of Muenster, Institute of Hygiene, Robert-Koch-Strasse 41, 48149, Muenster, Germany;University of Muenster, Department of Periodontology, Waldeyerstrasse 30, 48149, Muenster, Germany;Bundeswehr, Institute of Microbiology, Neuherbergstrasse 12, 80937, Muenchen, Germany;Rostock University Hospital, Institute of Medical Microbiology, Virology and Hygiene, Schillingallee 70, 18057 Rostock, Germany;University of Muenster, Department of Periodontology, Waldeyerstrasse 30, 48149, Muenster, Germany

Recommended Citation:
Markus H. Antwerpen,Karola Prior,Alexander Mellmann,et al. Rapid High Resolution Genotyping of Francisella tularensis by Whole Genome Sequence Comparison of Annotated Genes (“MLST+”)[J]. PLOS ONE,2015-01-01,10(4)
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