gchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0133047
Title:
Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France
Author: Gabriela Certad; Jean Dupouy-Camet; Nausicaa Gantois; Ourida Hammouma-Ghelboun; Muriel Pottier; Karine Guyot; Sadia Benamrouz; Marwan Osman; Baptiste Delaire; Colette Creusy; Eric Viscogliosi; Eduardo Dei-Cas; Cecile Marie Aliouat-Denis; Jérôme Follet
Source Publication: PLOS ONE
ISSN: 1932-6203
Indexed By: SCI-E
Publishing Year: 2015
Date Published: 2015-7-27
Volume: 10, Issue:7
Language: 英语
Keyword: Cryptosporidium parvum ; Freshwater fish ; Cryptosporidium ; Stomach ; Gastrointestinal tract ; Marine fish ; Inflammation ; Lakes
Subject in Chinese: 湖泊
Subject: LAKES
English Abstract: Cryptosporidium, a protozoan parasite that can cause severe diarrhea in a wide range of vertebrates including humans, is increasingly recognized as a parasite of a diverse range of wildlife species. However, little data are available regarding the identification of Cryptosporidium species and genotypes in wild aquatic environments, and more particularly in edible freshwater fish. To evaluate the prevalence of Cryptosporidiumspp. in fish from Lake Geneva (Lac Léman) in France, 41 entire fish and 100 fillets (cuts of fish flesh) were collected from fishery suppliers around the lake. Nested PCR using degenerate primers followed by sequence analysis was used. Five fish species were identified as potential hosts of Cryptosporidium: Salvelinus alpinus, Esox lucius, Coregonus lavaretus, Perca fluviatilis, and Rutilus rutilus. The presence of Cryptosporidium spp. was found in 15 out of 41 fish (37%), distributed as follows: 13 (87%) C. parvum, 1 (7%) C. molnari, and 1 (7%) mixed infection (C. parvum and C. molnari). C. molnari was identified in the stomach, while C. parvum was found in the stomach and intestine. C. molnari was also detected in 1 out of 100 analyzed fillets. In order to identify Cryptosporidium subtypes, sequencing of the highly polymorphic 60-kDa glycoprotein (gp60) was performed. Among the C. parvum positive samples, three gp60 subtypes were identified: IIaA15G2R1, IIaA16G2R1, and IIaA17G2R1. Histological examination confirmed the presence of potential developmental stages of C. parvum within digestive epithelial cells. These observations suggest that C. parvum is infecting fish, rather than being passively carried. Since C. parvum is a zoonotic species, fish potentially contaminated by the same subtypes found in terrestrial mammals would be an additional source of infection for humans and animals, and may also contribute to the contamination of the environment with this parasite. Moreover, the risk of human transmission is strengthened by the observation of edible fillet contamination.
Related Link: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0133047&type=printable
Citation statistics:
被引频次[WOS]:11   [查看WOS记录]     [查看WOS中相关记录]
Document Type: 期刊论文
Identifier: http://119.78.100.177/globalchange/handle/2HF3EXSE/22088
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

Files in This Item:
File Name/ File Size Content Type Version Access License
journal.pone.0133047.PDF(1157KB)期刊论文作者接受稿开放获取View 联系获取全文

Affiliation: Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Université Paris Descartes, Assistance Publique Hôpitaux de Paris, Parasitologie-Mycologie, Hôpital Cochin, Paris, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Laboratoire de Biotechnologie et Gestion des Agents Pathogènes en Agriculture, Institut Supérieur d’Agriculture de Lille, Lille, France;Faculté de Pharmacie, Université de Lille, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Ecologie et Biodiversité, Faculté Libre des Sciences et Technologies de Lille, Université Catholique de Lille, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Laboratoire Microbiologie, Santé et Environnement, Centre AZM pour la Recherche en Biotechnologie et ses Applications, Université Libanaise, Tripoli, Lebanon;Service d’Anatomie et de Cytologie Pathologiques, Groupe Hospitalier de l’Université Catholique de Lille, Lille, France;Service d’Anatomie et de Cytologie Pathologiques, Groupe Hospitalier de l’Université Catholique de Lille, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Centre Hospitalier Régional et Universitaire de Lille, Université Lille Nord de France, Lille, France;Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDEEP), Centre d'Infection et d'Immunité de Lille (CIIL), Institut Pasteur de Lille, INSERM U1019, CNRS UMR 8402, Université de Lille, Lille, France;Faculté de Pharmacie, Université de Lille, Lille, France;Laboratoire de Biotechnologie et Gestion des Agents Pathogènes en Agriculture, Institut Supérieur d’Agriculture de Lille, Lille, France;Laboratoire BioMEMS, Univ.Lille, CNRS, ISEN, Univ.Valenciennes, UMR 8520, IEMN, Institut d'Electronique de Microélectronique et de Nanotechnologie, F 59 000, Lille, France

Recommended Citation:
Gabriela Certad,Jean Dupouy-Camet,Nausicaa Gantois,et al. Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France[J]. PLOS ONE,2015-01-01,10(7)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gabriela Certad]'s Articles
[Jean Dupouy-Camet]'s Articles
[Nausicaa Gantois]'s Articles
百度学术
Similar articles in Baidu Scholar
[Gabriela Certad]'s Articles
[Jean Dupouy-Camet]'s Articles
[Nausicaa Gantois]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gabriela Certad]‘s Articles
[Jean Dupouy-Camet]‘s Articles
[Nausicaa Gantois]‘s Articles
Related Copyright Policies
Null
收藏/分享
文件名: journal.pone.0133047.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.