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DOI:	10.1371/journal.pone.0142008
论文题名:	The Dual Challenges of Generality and Specificity When Developing Environmental DNA Markers for Species and Subspecies of Oncorhynchus
作者:	Taylor M. Wilcox; Kellie J. Carim; Kevin S. McKelvey; Michael K. Young; Michael K. Schwartz
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2015
发表日期:	2015-11-4
卷:	10, 期:11
语种:	英语
英文关键词:	Trout ; Fishes ; Invasive species ; Lakes ; Animal phylogenetics ; Polymerase chain reaction ; Salmon ; Taxonomy
英文摘要:	Environmental DNA (eDNA) sampling is a powerful tool for detecting invasive and native aquatic species. Often, species of conservation interest co-occur with other, closely related taxa. Here, we developed qPCR (quantitative PCR) markers which distinguish westslope cutthroat trout (Oncorhynchus clarkii lewsi), Yellowstone cutthroat trout (O. clarkii bouvieri), and rainbow trout (O. mykiss), which are of conservation interest both as native species and as invasive species across each other’s native ranges. We found that local polymorphisms within westslope cutthroat trout and rainbow trout posed a challenge to designing assays that are generally applicable across the range of these widely-distributed species. Further, poorly-resolved taxonomies of Yellowstone cutthroat trout and Bonneville cutthroat trout (O. c. utah) prevented design of an assay that distinguishes these recognized taxa. The issues of intraspecific polymorphism and unresolved taxonomy for eDNA assay design addressed in this study are likely to be general problems for closely-related taxa. Prior to field application, we recommend that future studies sample populations and test assays more broadly than has been typical of published eDNA assays to date.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0142008&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/22354
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0142008.PDF（392KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;University of Montana, Division of Biological Sciences, 32 Campus Dr., Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America

Recommended Citation:

Taylor M. Wilcox,Kellie J. Carim,Kevin S. McKelvey,et al. The Dual Challenges of Generality and Specificity When Developing Environmental DNA Markers for Species and Subspecies of Oncorhynchus[J]. PLOS ONE,2015-01-01,10(11)

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