globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0138432
论文题名:
Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
作者: Joel F. Gibson; Shadi Shokralla; Colin Curry; Donald J. Baird; Wendy A. Monk; Ian King; Mehrdad Hajibabaei
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2015
发表日期: 2015-10-21
卷: 10, 期:10
语种: 英语
英文关键词: Taxonomy ; Biodiversity ; Sequence databases ; DNA sequence analysis ; Polymerase chain reaction ; Delta ecosystems ; DNA sequencing ; Wetlands
英文摘要: Biodiversity metrics are critical for assessment and monitoring of ecosystems threatened by anthropogenic stressors. Existing sorting and identification methods are too expensive and labour-intensive to be scaled up to meet management needs. Alternately, a high-throughput DNA sequencing approach could be used to determine biodiversity metrics from bulk environmental samples collected as part of a large-scale biomonitoring program. Here we show that both morphological and DNA sequence-based analyses are suitable for recovery of individual taxonomic richness, estimation of proportional abundance, and calculation of biodiversity metrics using a set of 24 benthic samples collected in the Peace-Athabasca Delta region of Canada. The high-throughput sequencing approach was able to recover all metrics with a higher degree of taxonomic resolution than morphological analysis. The reduced cost and increased capacity of DNA sequence-based approaches will finally allow environmental monitoring programs to operate at the geographical and temporal scale required by industrial and regulatory end-users.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0138432&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/22360
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Biodiversity Institute of Ontario and Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada;Environment Canada, Canada Centre for Inland Waters, Burlington, Ontario, Canada;Biodiversity Institute of Ontario and Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada;Environment Canada, Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, Canada;Environment Canada, Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, Canada;Canadian Rivers Institute, University of New Brunswick, Fredericton, New Brunswick, Canada;Biodiversity Institute of Ontario and Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada;Biodiversity Institute of Ontario and Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada

Recommended Citation:
Joel F. Gibson,Shadi Shokralla,Colin Curry,et al. Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing[J]. PLOS ONE,2015-01-01,10(10)
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