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DOI: 10.1371/journal.pone.0154666
Title:
Discriminating Intercalative Effects of Threading Intercalator Nogalamycin, from Classical Intercalator Daunomycin, Using Single Molecule Atomic Force Spectroscopy
Author: T. Banerjee; S. Banerjee; S. Sett; S. Ghosh; T. Rakshit; R. Mukhopadhyay
Source Publication: PLOS ONE
ISSN: 1932-6203
Indexed By: SCI-E
Publishing Year: 2016
Date Published: 2016-5-16
Volume: 11, Issue:5
Language: 英语
Keyword: Drug therapy ; Melting ; DNA structure ; Absorption spectroscopy ; Chemical dissociation ; Atomic force microscopy ; Biopolymers ; Plateaus
Subject in Chinese: 吸收 ; 吸附作用
Subject: ABSORPTION
English Abstract: DNA threading intercalators are a unique class of intercalating agents, albeit little biophysical information is available on their intercalative actions. Herein, the intercalative effects of nogalamycin, which is a naturally-occurring DNA threading intercalator, have been investigated by high-resolution atomic force microscopy (AFM) and spectroscopy (AFS). The results have been compared with those of the well-known chemotherapeutic drug daunomycin, which is a non-threading classical intercalator bearing structural similarity to nogalamycin. A comparative AFM assessment revealed a greater increase in DNA contour length over the entire incubation period of 48 h for nogalamycin treatment, whereas the contour length increase manifested faster in case of daunomycin. The elastic response of single DNA molecules to an externally applied force was investigated by the single molecule AFS approach. Characteristic mechanical fingerprints in the overstretching behaviour clearly distinguished the nogalamycin/daunomycin-treated dsDNA from untreated dsDNA—the former appearing less elastic than the latter, and the nogalamycin-treated DNA distinguished from the daunomycin-treated DNA—the classically intercalated dsDNA appearing the least elastic. A single molecule AFS-based discrimination of threading intercalation from the classical type is being reported for the first time.
Related Link: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0154666&type=printable
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被引频次[WOS]:5   [查看WOS记录]     [查看WOS中相关记录]
Document Type: 期刊论文
Identifier: http://119.78.100.177/globalchange/handle/2HF3EXSE/23647
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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Affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India;Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India;Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India;Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India;Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India;Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, 700 032, India

Recommended Citation:
T. Banerjee,S. Banerjee,S. Sett,et al. Discriminating Intercalative Effects of Threading Intercalator Nogalamycin, from Classical Intercalator Daunomycin, Using Single Molecule Atomic Force Spectroscopy[J]. PLOS ONE,2016-01-01,11(5)
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