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DOI:	10.1371/journal.pone.0163031
论文题名:	Inability of ‘Whole Genome Amplification’ to Improve Success Rates for the Biomolecular Detection of Tuberculosis in Archaeological Samples
作者:	Jannine Forst; Terence A. Brown
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2016
发表日期:	2016-9-21
卷:	11, 期:9
语种:	英语
英文关键词:	Polymerase chain reaction ; Ribs ; Ancient DNA ; DNA extraction ; Sequence databases ; Vertebrae ; Archaeology ; Skeleton
英文摘要:	We assessed the ability of whole genome amplification (WGA) to improve the efficiency of downstream polymerase chain reactions (PCRs) directed at ancient DNA (aDNA) of members of the Mycobacterium tuberculosis complex (MTBC). Using extracts from a variety of bones and a tooth from human skeletons with or without lesions indicative of tuberculosis, from multiple time periods, we obtained inconsistent results. We conclude that WGA does not provide any advantage in studies of MTBC aDNA. The sporadic nature of our results are probably due to the fact that WGA is itself a PCR-based procedure which, although designed to deal with fragmented DNA, might be inefficient with the low concentration of templates in an aDNA extract. As such, WGA is subject to similar, if not the same, restrictions as PCR when applied to aDNA.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0163031&type=printable
Citation statistics:	被引频次[WOS]:2 [查看WOS记录] [查看WOS中相关记录]
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/23916
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0163031.PDF（2002KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Manchester Institute of Biotechnology, School of Earth and Environmental Sciences, University of Manchester, Manchester, United Kingdom;Manchester Institute of Biotechnology, School of Earth and Environmental Sciences, University of Manchester, Manchester, United Kingdom

Recommended Citation:

Jannine Forst,Terence A. Brown. Inability of ‘Whole Genome Amplification’ to Improve Success Rates for the Biomolecular Detection of Tuberculosis in Archaeological Samples[J]. PLOS ONE,2016-01-01,11(9)

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