globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0145558
论文题名:
Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes
作者: Corinne Biderre-Petit; Eric Dugat-Bony; Mickaël Mege; Nicolas Parisot; Lorenz Adrian; Anne Moné; Jérémie Denonfoux; Eric Peyretaillade; Didier Debroas; Delphine Boucher; Pierre Peyret
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2016
发表日期: 2016-1-6
卷: 11, 期:1
语种: 英语
英文关键词: Lakes ; Ribosomal RNA ; Polymerase chain reaction ; Surface water ; Water columns ; Genomic databases ; Genomic library construction ; Sequence assembly tools
英文摘要: Here we describe the natural occurrence of bacteria of the class Dehalococcoidia (DEH) and their diversity at different depths in anoxic waters of a remote meromictic lake (Lake Pavin) using 16S rRNA gene amplicon sequencing and quantitative PCR. Detected DEH are phylogenetically diverse and the majority of 16S rRNA sequences have less than 91% similarity to previously isolated DEH 16S rRNA sequences. To predict the metabolic potential of detected DEH subgroups and to assess if they encode genes to transform halogenated compounds, we enriched DEH-affiliated genomic DNA by using a specific-gene capture method and probes against DEH-derived 16S rRNA genes, reductive dehalogenase genes and known insertion sequences. Two reductive dehalogenase homologous sequences were identified from DEH-enriched genomic DNA, and marker genes in the direct vicinity confirm that gene fragments were derived from DEH. The low sequence similarity with known reductive dehalogenase genes suggests yet-unknown catabolic potential in the anoxic zone of Lake Pavin.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145558&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/25000
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Laboratoire “Microorganismes: Génome et Environnement,” Clermont Université, Université Blaise Pascal, F-63000, Clermont-Ferrand, France;Laboratoire Microorganismes, Génome et Environnement, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6023, F-63171, Aubière, France;UMR GMPA, AgroParisTech, INRA, Université Paris-Saclay, 78850, Thiverval-Grignon, France;Laboratoire “Microorganismes: Génome et Environnement,” Clermont Université, Université Blaise Pascal, F-63000, Clermont-Ferrand, France;Laboratoire Microorganismes, Génome et Environnement, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6023, F-63171, Aubière, France;Laboratoire “Microorganismes: Génome et Environnement,” Clermont Université, Université Blaise Pascal, F-63000, Clermont-Ferrand, France;Laboratoire Microorganismes, Génome et Environnement, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6023, F-63171, Aubière, France;Clermont Université, Université d’Auvergne, EA 4678 CIDAM, BP 10448, F-63001, Clermont-Ferrand, France;Helmholtz Centre for Environmental Research–UFZ, Permoserstraße 15, D-04318, Leipzig, Germany;Laboratoire “Microorganismes: Génome et Environnement,” Clermont Université, Université Blaise Pascal, F-63000, Clermont-Ferrand, France;Laboratoire Microorganismes, Génome et Environnement, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6023, F-63171, Aubière, France;Clermont Université, Université d’Auvergne, EA 4678 CIDAM, BP 10448, F-63001, Clermont-Ferrand, France;Clermont Université, Université d’Auvergne, EA 4678 CIDAM, BP 10448, F-63001, Clermont-Ferrand, France;Laboratoire “Microorganismes: Génome et Environnement,” Clermont Université, Université Blaise Pascal, F-63000, Clermont-Ferrand, France;Laboratoire Microorganismes, Génome et Environnement, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6023, F-63171, Aubière, France;Clermont Université, Université d’Auvergne, EA 4678 CIDAM, BP 10448, F-63001, Clermont-Ferrand, France;Clermont Université, Université d’Auvergne, EA 4678 CIDAM, BP 10448, F-63001, Clermont-Ferrand, France

Recommended Citation:
Corinne Biderre-Petit,Eric Dugat-Bony,Mickaël Mege,et al. Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes[J]. PLOS ONE,2016-01-01,11(1)
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