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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0169334
论文题名:	A Noninvasive Tool to Assess the Distribution of Pacific Lamprey (Entosphenus tridentatus) in the Columbia River Basin
作者:	Kellie J. Carim; J. Caleb Dysthe; Michael K. Young; Kevin S. McKelvey; Michael K. Schwartz
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2017
发表日期:	2017-1-9
卷:	12, 期:1
语种:	英语
英文关键词:	Lampreys ; Trout ; Salmon ; Polymerase chain reaction ; DNA sequence analysis ; Sequence alignment ; Freshwater fish ; Rivers
英文摘要:	The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish once abundant throughout coastal basins of western North America that has suffered dramatic declines in the last century due primarily to human activities. Here, we describe the development of an environmental DNA (eDNA) assay to detect Pacific lamprey in the Columbia River basin. The eDNA assay successfully amplified tissue derived DNA of Pacific lamprey collected from 12 locations throughout the Columbia River basin. The assay amplifies DNA from other Entosphenus species found outside of the Columbia River basin, but is species-specific within this basin. As a result, the assay presented here may be useful for detecting Entosphenus spp. in geographic range beyond the Columbia River Basin. The assay did not amplify tissue or synthetically derived DNA of 14 commonly sympatric non-target species, including lampreys of the genus Lampetra, which are morphologically similar to Pacific lamprey in the freshwater larval stage.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169334&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25778
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0169334.pdf（1136KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America;United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America

Recommended Citation:

Kellie J. Carim,J. Caleb Dysthe,Michael K. Young,et al. A Noninvasive Tool to Assess the Distribution of Pacific Lamprey (Entosphenus tridentatus) in the Columbia River Basin[J]. PLOS ONE,2017-01-01,12(1)

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