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DOI:	10.1371/journal.pone.0169190
论文题名:	Physiological, Biochemical, and Biophysical Characterization of the Lung-Lavaged Spontaneously-Breathing Rabbit as a Model for Respiratory Distress Syndrome
作者:	Francesca Ricci; Chiara Catozzi; Xabier Murgia; Brenda Rosa; Davide Amidani; Luca Lorenzini; Federico Bianco; Claudio Rivetti; Silvia Catinella; Gino Villetti; Maurizio Civelli; Barbara Pioselli; Carlo Dani; Fabrizio Salomone
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2017
发表日期:	2017-1-6
卷:	12, 期:1
语种:	英语
英文关键词:	Surfactants ; Phospholipids ; Rabbits ; Respiratory physiology ; Liquid chromatography-mass spectrometry ; Intubation ; Isotherms ; Blood
英文摘要:	Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of non-invasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS). Surfactant administration techniques compatible with nCPAP ventilation strategy are actively investigated. Our aim is to set up and validate a respiratory distress animal model that can be managed on nCPAP suitable for surfactant administration techniques studies. Surfactant depletion was induced by bronchoalveolar lavages (BALs) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BALs samples. Animals were randomized into two groups: Control group (nCPAP only) and InSurE group, consisting of a bolus of surfactant (Poractant alfa, 200 mg/kg) followed by nCPAP. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated just before and after BALs, at the time of treatment, and at the end of the procedure. Surfactant phospholipids and protein analysis as well as surface tension measurements on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE group showed a significant improvement of blood oxygenation and lung mechanics. On the contrary, no signs of recovery were appreciated in animals treated with just nCPAP. The surfactant-depleted adult rabbit RDS model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population is of particular interest as potential target for the non-invasive administration of surfactant.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169190&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25962
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0169190.pdf（1982KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Chiesi Farmaceutici, R&D Department, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany;Chiesi Farmaceutici, R&D Department, Parma, Italy;Department of Life Sciences, University of Parma, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Department of Life Sciences, University of Parma, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy;Department of Neurosciences, Psychology, Drug Research and Child Health, Careggi University Hospital of Florence, Florence, Italy;Chiesi Farmaceutici, R&D Department, Parma, Italy

Recommended Citation:

Francesca Ricci,Chiara Catozzi,Xabier Murgia,et al. Physiological, Biochemical, and Biophysical Characterization of the Lung-Lavaged Spontaneously-Breathing Rabbit as a Model for Respiratory Distress Syndrome[J]. PLOS ONE,2017-01-01,12(1)

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