全球变化知识资源中心

Knowledge Resources Center for Global Change


Email:
Passwd
验证:
	换一张
Have you forgotten your password? Stay signed in Log In

globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0168787
论文题名:	Environmental DNA Detection of the Golden Tree Frog (Phytotriades auratus) in Bromeliads
作者:	Jack D. Torresdal; Aidan D. Farrell; Caren S. Goldberg
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2017
发表日期:	2017-1-4
卷:	12, 期:1
语种:	英语
英文关键词:	Frogs ; Trees ; Polymerase chain reaction ; Amphibians ; DNA filter assay ; Forests ; DNA analysis ; Fresh water
英文摘要:	The analysis of environmental DNA (eDNA) is a powerful, non-destructive technique for detecting rare or hard to find freshwater organisms. In this study, we investigated the effectiveness of environmental DNA analysis as a method for detecting a rare amphibian, the golden tree frog (Phytotriades auratus). These frogs are believed to live exclusively within one species of tank bromeliad, Glomeropitcairnia erectiflora, found on the highest peaks of the island of Trinidad in the West Indies. Previous survey methods for this species involved bromeliad destruction, while here we collected and analyzed water samples from discrete pools within G. erectiflora plants for species-specific DNA. We found 1) that we can identify the presence of P. auratus in the bromeliads using environmental DNA analysis, and 2) that environmental DNA evidence indicates the presence of a previously undiscovered P. auratus population, increasing the species’ range from two isolated ‘sky islands’ to three.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0168787&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/25964
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

Files in This Item:	Download All

File Name/ File Size	Content Type	Version	Access	License
journal.pone.0168787.pdf（827KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Independent Scholar, Portland, Oregon, United States of America;Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago;School of the Environment, Washington State University, Pullman, Washington, United States of America

Recommended Citation:

Jack D. Torresdal,Aidan D. Farrell,Caren S. Goldberg. Environmental DNA Detection of the Golden Tree Frog (Phytotriades auratus) in Bromeliads[J]. PLOS ONE,2017-01-01,12(1)

Service
	Recommend this item
	Sava as my favorate item
	Show this item's statistics
	Export Endnote File
Google Scholar
	Similar articles in Google Scholar
	[Jack D. Torresdal]'s Articles
	[Aidan D. Farrell]'s Articles
	[Caren S. Goldberg]'s Articles
百度学术
	Similar articles in Baidu Scholar
	[Jack D. Torresdal]'s Articles
	[Aidan D. Farrell]'s Articles
	[Caren S. Goldberg]'s Articles
CSDL cross search
	Similar articles in CSDL Cross Search
	[Jack D. Torresdal]‘s Articles
	[Aidan D. Farrell]‘s Articles
	[Caren S. Goldberg]‘s Articles
Related Copyright Policies
Null
收藏/分享