| DOI: | 10.2172/1010281
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| 报告号: | 45234
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| 报告题名: | Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site |
| 作者: | Moser, Duane P.; Bruckner, Jim; Fisher, Jen; Czerwinski, Ken; Russell, Charles E.; Zavarin, Mavrik
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| 出版年: | 2010
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| 发表日期: | 2010-09-01
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site
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| 英文摘要: | This U.S. Department of Energy (DOE) Environmental Remediation Sciences Project (ERSP) was designed to test fundamental hypotheses concerning the existence and nature of indigenous microbial populations of Nevada Test Site subsurface nuclear test/detonation cavities. Now called Subsurface Biogeochemical Research (SBR), this programâs Exploratory Research (ER) element, which funded this research, is designed to support high risk, high potential reward projects. Here, five cavities (GASCON, CHANCELLOR, NASH, ALEMAN, and ALMENDRO) and one tunnel (U12N) were sampled using bailers or pumps. Molecular and cultivation-based techniques revealed bacterial signatures at five sites (CHANCELLOR may be lifeless). SSU rRNA gene libraries contained diverse and divergent microbial sequences affiliated with known metal- and sulfur-cycling microorganisms, organic compound degraders, microorganisms from deep mines, and bacteria involved in selenate reduction and arsenite oxidation. Close relatives of Desulforudis audaxviator, a microorganism thought to subsist in the terrestrial deep subsurface on H2 and SO42- produced by radiochemical reactions, was detected in the tunnel waters. NTS-specific media formulations were used to culture and quantify nitrate-, sulfate-, iron-reducing, fermentative, and methanogenic microorganisms. Given that redox manipulations mediated by microorganisms can impact the mobility of DOE contaminants, our results should have implications for management strategies at this and other DOE sites. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1010281
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/39695
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1010281.pdf（401KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Moser, Duane P.,Bruckner, Jim,Fisher, Jen,et al. Characterization of Microbial Communities in Subsurface Nuclear Blast Cavities of the Nevada Test Site. 2010-01-01.
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