| DOI: | 10.2172/986219
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| 报告号: | LBNL-3803E-Poster
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| 报告题名: | High Throughput Identification, Purification and Structural Characterization of Water Soluble Protein Complexes in Desulfovibrio vulgaris |
| 作者: | Dong,, Ming; Han, Bong-Gyoon; Liu, Hui-Hai; Malik, J.; Geller, Jil; Yang, Li; Choi, M.; Chandonia, John-Marc; Arbelaez, Pablo; Sterling, H. J.; Typke, Dieter; Shatsky, Max; Brenner, Steve; Fisher, Susan; Williams, Evan; Szakal, Evelin; Allen, S.; Hall, S.
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| 出版年: | 2010
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| 发表日期: | 2010-05-17
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| 总页数: | 1
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | tagless purification of water soluble complexes
; chromatography
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| 中文主题词: | 降水
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| 主题词: | PRECIPITATION
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| 英文摘要: | Our scheme for the tagless purification of water soluble complexes. 10 g of protein from a crude bacterial extract is first fractionated by ammonium sulfate precipitation and then by a series of chromatographic steps: anion exchange (IEX), hydrophobic interaction (HIC), and finally size exclusion (Gel Filtration). Fractions from the last chromatography step are trypsin digested and peptides labeled with iTRAQ reagents to allow multiplexing and quantitation during mass spectrometric analysis. Elution profiles of identified proteins are then subjected to clustering analysis. |
| URL: | http://www.osti.gov/scitech/servlets/purl/986219
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/39809
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 986219.pdf（4876KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Dong,, Ming,Han, Bong-Gyoon,Liu, Hui-Hai,et al. High Throughput Identification, Purification and Structural Characterization of Water Soluble Protein Complexes in Desulfovibrio vulgaris. 2010-01-01.
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