| DOI: | 10.2172/986222
|
| 报告号: | LBNL-3800E-Poster
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| 报告题名: | GeoChip-based Analysis of Groundwater Microbial Diversity in Norman Landfill |
| 作者: | Lu, Zhenmei; He, Zhili; Parisi, Victoria; Kang, Sanghoon; Deng, Ye; Nostrand, Joy Van; Masoner, Jason; Cozzarelli, Isabelle; Suflita, Joseph; Zhou, Jizhong
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| 出版年: | 2010
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| 发表日期: | 2010-05-17
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| 总页数: | 1
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Norman Landfill
; leachate from unlined landfills
; groundwater microbial communities
|
| 中文主题词: | 碳
; 氮
; 氨
; 有机碳
; 污染物
|
| 主题词: | CARBON
; NITROGEN
; AMMONIA
; ORGANIC CARBON
; CONTAMINANTS
|
| 英文摘要: | The Norman Landfill is a closed municipal solid waste landfill located on an alluvium associated with the Canadian River in Norman, Oklahoma. It has operated as a research site since 1994 because it is typical of many closed landfill sites across the U.S. Leachate from the unlined landfill forms a groundwater plume that extends downgradient approximately 250 m from the landfill toward the Canadian River. To investigate the impact of the landfill leachate on the diversity and functional structure of microbial communities, groundwater samples were taken from eight monitoring wells at a depth of 5m, and analyzed using a comprehensive functional gene array covering about 50,000 genes involved in key microbial processes, such as biogeochemical cycling of C, N, P, and S, and bioremediation of organic contaminants and metals. Wells are located within a transect along a presumed flow path with different distances to the center of the leachate plume. Our analyses showed that microbial communities were obviously impacted by the leachate-component from the landfill. The number of genes detected and microbial diversity indices in the center (LF2B) and its closest (MLS35) wells were significantly less than those detected in other more downgradient wells, while no significant changes were observed in the relative abundance (i.e., percentage of each gene category) for most gene categories. However, the microbial community composition or structure of the landfill groundwater did not clearly show a significant correlation with the distance from well LF2B. Burkholderia sp. and Pseudomonas sp. were found to be the dominant microbial populations detected in all wells, while Bradyrhizobium sp. and Ralstonia sp. were dominant populations for seven wells except LF2B. In addition, Mantel test and canonical correspondence analysis (CCA) indicate that pH, sulfate, ammonia nitrogen and dissolved organic carbon (DOC) have significant effects on the microbial community structure. The results suggest that the leachate from unlined landfills significantly impact the structures of groundwater microbial communities, and that more distal wells recover by natural attenuation. |
| URL: | http://www.osti.gov/scitech/servlets/purl/986222
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/39811
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| File Name/ File Size |
Content Type |
Version |
Access |
License |
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| 986222.pdf（562KB） | 研究报告 | -- | 开放获取 | | View
Download
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| Recommended Citation: | |
Lu, Zhenmei,He, Zhili,Parisi, Victoria,et al. GeoChip-based Analysis of Groundwater Microbial Diversity in Norman Landfill. 2010-01-01.
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