DOI: | 10.2172/1004597
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报告号: | LBNL-4224E-Poster
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报告题名: | Development of Extraction Techniques for the Detection of Signature Lipids from Oil |
作者: | Borglin, Sharon; Geller, Jil; Chakraborty, Romy; Hazen, Terry; Mason, Olivia
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出版年: | 2010
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发表日期: | 2010-05-17
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总页数: | 1
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国家: | 美国
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语种: | 英语
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英文关键词: | Desulfovibrio vulgaris
; Methanococcus maripaludus
; recovery of lipids
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英文摘要: | Pure cultures, including Desulfovibrio vulgaris and Methanococcus maripaludus, were combined with model oil samples and oil/diesel mixtures to optimize extraction techniques of signature lipids from oil in support of investigation of microbial communities in oil deposit samples targets for microbial enhanced hydrocarbon recovery. Several techniques were evaluated, including standard phospholipid extraction, ether linked lipid for Archaeal bacterial detection, and high pressure extractiontechniques. Recovery of lipids ranged from 50-80percent as compared to extraction of the pure culture. Extraction efficiency was evaluated by the use of internal standards. Field samples will also be tested for recovery of signature lipids with optimized extraction techniques. |
URL: | http://www.osti.gov/scitech/servlets/purl/1004597
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资源类型: | 研究报告
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标识符: | http://119.78.100.158/handle/2HF3EXSE/39818
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Appears in Collections: | 过去全球变化的重建 影响、适应和脆弱性 科学计划与规划 气候变化与战略 全球变化的国际研究计划 气候减缓与适应 气候变化事实与影响
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1004597.pdf(719KB) | 研究报告 | -- | 开放获取 | | View
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Recommended Citation: |
Borglin, Sharon,Geller, Jil,Chakraborty, Romy,et al. Development of Extraction Techniques for the Detection of Signature Lipids from Oil. 2010-01-01.
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