| DOI: | 10.2172/1077002
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| 报告号: | DOE-GEORGIA TECH RES CORP-64124
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| 报告题名: | MOLECULAR MECHANISM OF MICROBIAL TECHNETIUM REDUCTION FINAL REPORT |
| 作者: | DiChristina, Thomas J. [Georgia Tech]
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| 出版年: | 2013
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| 发表日期: | 2013-04-30
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | Microbial technetium reduction
; bioremediation
; technetium-contaminated subsurface environments
; in situ remediation strategy
; molecular mechanism
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| 中文主题词: | 离子
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| 主题词: | IONS
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| 英文摘要: | Microbial Tc(VII) reduction is an attractive alternative strategy for bioremediation of technetium-contaminated subsurface environments. Traditional ex situ remediation processes (e.g., adsorption or ion exchange) are often limited by poor extraction efficiency, inhibition by competing ions and production of large volumes of produced waste. Microbial Tc(VII) reduction provides an attractive alternative in situ remediation strategy since the reduced end-product Tc(IV) precipitates as TcO2, a highly insoluble hydrous oxide. Despite its potential benefits, the molecular mechanism of microbial Tc(VII) reduction remains poorly understood. The main goal of the proposed DOENABIR research project is to determine the molecular mechanism of microbial Tc(VII) reduction. Random mutagenesis studies in our lab have resulted in generation of a set of six Tc(VII) reduction-deficient mutants of Shewanella oneidensis. The anaerobic respiratory deficiencies of each Tc(VII) reduction-deficient mutant was determined by anaerobic growth on various combinations of three electron donors and 14 terminal electron acceptors. Results indicated that the electron transport pathways to Tc(VII), NO3 -, Mn(III) and U(VI) share common structural or regulatory components. In addition, we have recently found that wild-type Shewanella are also able to reduce Tc(IV) as electron acceptor, producing Tc(III) as an end-product. The recent genome sequencing of a variety of technetium-reducing bacteria and the anticipated release of several additional genome sequences in the coming year, provides us with an unprecedented opportunity to determine the mechanism of microbial technetium reduction across species and genus lines. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1077002
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/41164
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1077002.pdf（69KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
DiChristina, Thomas J. [Georgia Tech]. MOLECULAR MECHANISM OF MICROBIAL TECHNETIUM REDUCTION FINAL REPORT. 2013-01-01.
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