| DOI: | 10.2172/1275739
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| 报告号: | DOE--Caltech-0003940
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| 报告题名: | Integrative molecular and microanalytical studies of syntrophic partnerships linking C, S, and N cycles in anoxic environments |
| 作者: | Orphan, Victoria
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| 出版年: | 2016
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| 发表日期: | 2016-07-15
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| 总页数: | 6
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| 国家: | 美国
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| 语种: | 英语
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| 英文关键词: | stable isotope probing
; nanoSIMS
; anaerobic oxidation of methane
; fluorescence in situ hybridization
; archaea
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| 中文主题词: | 碳
; 荧光
; 甲烷
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| 主题词: | CARBON
; FLUORESCENCE
; METHANE
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| 英文摘要: | Syntrophy and other forms of symbiotic associations between microorganisms are central to carbon and nutrient cycling in the environment. However, the inherent interdependence of these interactions, dynamic behavior, and frequent existence at thermodynamic limits has hindered our ability to both recognize syntrophic partnerships in nature and effectively study their behavior in the laboratory. To characterize and understand the underlying factors influencing syntrophic associations within complex communities requires a suite of tools that extend beyond basic molecular identification and cultivation. This specifically includes methods that preserve the natural spatial relationships between metabolically interdependent microorganisms while allowing downstream geochemical and/or molecular analysis. With support from this award, we have developed and applied new combinations of molecular, microscopy, and stable isotope-based methodologies that enable the characterization of fundamental links between phylogenetically-identified microorganisms and their specific metabolic activities and interactions in the environment. Through the coupling of fluorescence in situ hybridization (FISH) with cell capture and targeted metagenomics (Magneto-FISH), and FISH + secondary ion mass spectrometry (i.e. FISH-SIMS or FISH-nanoSIMS), we have defined new microbial interactions and the ecophysiology of anaerobic microorganisms involved in environmental methane cycling. |
| URL: | http://www.osti.gov/scitech/servlets/purl/1275739
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| Citation statistics: |
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| 资源类型: | 研究报告
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/42062
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| Appears in Collections: | 过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响
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| 1275739.pdf（859KB） | 研究报告 | -- | 开放获取 | | View
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| Recommended Citation: | |
Orphan, Victoria. Integrative molecular and microanalytical studies of syntrophic partnerships linking C, S, and N cycles in anoxic environments. 2016-01-01.
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