globalchange  > 过去全球变化的重建
DOI: 10.1016/j.quascirev.2013.11.007
Scopus记录号: 2-s2.0-84889576365
论文题名:
Thorough assessment of DNA preservation from fossil bone and sediments excavated from a late Pleistocene-Holocene cave deposit on Kangaroo Island, South Australia
作者: Haouchar D.; Haile J.; McDowell M.C.; Murray D.C.; White N.E.; Allcock R.J.N.; Phillips M.J.; Prideaux G.J.; Bunce M.
刊名: Quaternary Science Reviews
ISSN: 2773791
出版年: 2014
卷: 84
起始页码: 56
结束页码: 64
语种: 英语
英文关键词: Ancient DNA ; Biodiversity ; Fossils ; Pleistocene-Holocene ; Quaternary
Scopus关键词: Biodiversity ; Bone ; Caves ; DNA ; Extraction ; Gene encoding ; Sediments ; Stratigraphy ; Ancient dnas ; Biological information ; Environmental change ; Extraction techniques ; Fossils ; Late Pleistocene-Holocene ; Pleistocene-Holocene ; Quaternary ; Biological materials preservation ; biostratigraphy ; bone ; cave deposit ; climate variation ; fossil record ; Holocene ; marsupial ; mitochondrial DNA ; morphology ; paleoenvironment ; paleontology ; Pleistocene ; preservation ; reptile ; Australia ; Kangaroo Island ; South Australia ; Eutheria ; Metatheria ; Reptilia ; Vertebrata
英文摘要: Fossils and sediments preserved in caves are an excellent source of information for investigating impacts of past environmental changes on biodiversity. Until recently studies have relied on morphology-based palaeontological approaches, but recent advances in molecular analytical methods offer excellent potential for extracting a greater array of biological information from these sites. This study presents a thorough assessment of DNA preservation from late Pleistocene-Holocene vertebrate fossils and sediments from Kelly Hill Cave Kangaroo Island, South Australia. Using a combination of extraction techniques and sequencing technologies, ancient DNA was characterised from over 70 bones and 20 sediment samples from 15 stratigraphic layers ranging in age from >20ka to ~6.8ka. A combination of primers targeting marsupial and placental mammals, reptiles and two universal plant primers were used to reveal genetic biodiversity for comparison with the mainland and with the morphological fossil record for Kelly Hill Cave. We demonstrate that Kelly Hill Cave has excellent long-term DNA preservation, back to at least 20ka. This contrasts with the majority of Australian cave sites thus far explored for ancient DNA preservation, and highlights the great promise Kangaroo Island caves hold for yielding the hitherto-elusive DNA of extinct Australian Pleistocene species. © 2013 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/60399
Appears in Collections:过去全球变化的重建

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作者单位: Australian Wildlife Forensic Services and Ancient DNA Laboratory, School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA 6150, Australia; School of Biological Sciences, Flinders University, Bedford Park, SA 5042, Australia; LotteryWest State Biomedical Facility: Genomics, School of Pathology and Laboratory Medicine, The University of Western Australia, Nedlands, WA 6009, Australia; School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia; Trace and Environmental DNA Laboratory, Department of Environment and Agriculture, Curtin University, Perth, WA 6845, Australia

Recommended Citation:
Haouchar D.,Haile J.,McDowell M.C.,et al. Thorough assessment of DNA preservation from fossil bone and sediments excavated from a late Pleistocene-Holocene cave deposit on Kangaroo Island, South Australia[J]. Quaternary Science Reviews,2014-01-01,84
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