DOI: 10.1016/j.quascirev.2013.06.006
Scopus记录号: 2-s2.0-84880356631
论文题名: A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa
作者: Pedersen M.W. ; Ginolhac A. ; Orlando L. ; Olsen J. ; Andersen K. ; Holm J. ; Funder S. ; Willerslev E. ; Kjær K.H.
刊名: Quaternary Science Reviews
ISSN: 2773791
出版年: 2013
卷: 75 起始页码: 161
结束页码: 168
语种: 英语
英文关键词: ADNA
; Lake sediments
; Metabarcode
; SedaDNA
; TrnL
Scopus关键词: ADNA
; Lake sediments
; Metabarcode
; SedaDNA
; TrnL
; Aquatic organisms
; DNA
; Gene encoding
; Lakes
; Sediments
; Plants (botany)
; chronology
; comparative study
; floristics
; fossil record
; genetic analysis
; lacustrine deposit
; paleobotany
; phylogenetics
; plant community
; pollen
; radiocarbon dating
; taxonomy
; Arctic
; Greenland
英文摘要: We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa. © 2013 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/60554
Appears in Collections: 过去全球变化的重建
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作者单位: Centre for GeoGenetics, Natural History Museum of Denmark, Øster Voldgade 5-7, 1350 Copenhagen K, Denmark; Department of Physics and Astronomy, Aarhus University, Ny Munkegade 120, 8000 Aarhus, Denmark
Recommended Citation:
Pedersen M.W.,Ginolhac A.,Orlando L.,et al. A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa[J]. Quaternary Science Reviews,2013-01-01,75