globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gcb.13790
论文题名:
Differential sensitivity of total and active soil microbial communities to drought and forest management
作者: Bastida F.; Torres I.F.; Andrés-Abellán M.; Baldrian P.; López-Mondéjar R.; Větrovský T.; Richnow H.H.; Starke R.; Ondoño S.; García C.; López-Serrano F.R.; Jehmlich N.
刊名: Global Change Biology
ISSN: 13541013
出版年: 2017
卷: 23, 期:10
起始页码: 4185
结束页码: 4203
语种: 英语
英文关键词: climate change ; drought ; forest management ; genomics ; metaproteomics ; microbial biomass ; microbial community ; semiarid
Scopus关键词: Bacteria (microorganisms) ; Pinus halepensis
英文摘要: Climate change will affect semiarid ecosystems through severe droughts that increase the competition for resources in plant and microbial communities. In these habitats, adaptations to climate change may consist of thinning—that reduces competition for resources through a decrease in tree density and the promotion of plant survival. We deciphered the functional and phylogenetic responses of the microbial community to 60 years of drought induced by rainfall exclusion and how forest management affects its resistance to drought, in a semiarid forest ecosystem dominated by Pinus halepensis Mill. A multiOMIC approach was applied to reveal novel, community-based strategies in the face of climate change. The diversity and the composition of the total and active soil microbiome were evaluated by 16S rRNA gene (bacteria) and ITS (fungal) sequencing, and by metaproteomics. The microbial biomass was analyzed by phospholipid fatty acids (PLFAs), and the microbially mediated ecosystem multifunctionality was studied by the integration of soil enzyme activities related to the cycles of C, N, and P. The microbial biomass and ecosystem multifunctionality decreased in drought-plots, as a consequence of the lower soil moisture and poorer plant development, but this decrease was more notable in unthinned plots. The structure and diversity of the total bacterial community was unaffected by drought at phylum and order level, but did so at genus level, and was influenced by seasonality. However, the total fungal community and the active microbial community were more sensitive to drought and were related to ecosystem multifunctionality. Thinning in plots without drought increased the active diversity while the total diversity was not affected. Thinning promoted the resistance of ecosystem multifunctionality to drought through changes in the active microbial community. The integration of total and active microbiome analyses avoids misinterpretations of the links between the soil microbial community and climate change. © 2017 John Wiley & Sons Ltd
资助项目: The authors thank the Spanish Ministry for the MINECO AGL2014-54636-R and AGL2014-55658-R. Felipe Bastida thanks the Spanish Government for his “Ramón y Cajal” contract and FEDER funds (RYC-2012-10666). The authors are grateful to the “CSIC-University of Castilla La Mancha Associated Unit” and to the Fundación Séneca (19896/GERM/15). P. Baldrian, T. Větrovský, and R. López Mondéjar were supported by the research concept of the Institute of Microbiology of the CAS (RVO61388971) and by C4Sys (LM2015055). N.J. was partially funded by SFB Aquadiva. The authors are grateful for the use of the analytical facilities of the Centre for Chemical Microscopy (ProVIS) at the Helmholtz Centre for Environmental Research, which is supported by European Regional Development Funds (EFRE—Europe funds Saxony) and the Helmholtz Association. The authors are grateful to Ivan Prieto for his statistical advice. Benjamin Scheer (Department of Isotope Biogeochemistry) is acknowledged for technical support during protein analysis in the ProVIS laboratory. Kathleen Eismann (Department of Molecular Systems Biology) is acknowledged for technical support in proteome sample preparation.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/60795
Appears in Collections:影响、适应和脆弱性

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作者单位: Department of Soil and Water Conservation, CEBAS-CSIC, Murcia, Spain; Department of Science and Agroforestry Technology and Genetics, Higher Technical School of Agricultural and Forestry Engineering, University of Castilla-La Mancha, Albacete, Spain; Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Praha 4, Czech Republic; Department of Isotope Biogeochemistry, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany; Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany

Recommended Citation:
Bastida F.,Torres I.F.,Andrés-Abellán M.,et al. Differential sensitivity of total and active soil microbial communities to drought and forest management[J]. Global Change Biology,2017-01-01,23(10)
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