DOI: 10.1016/j.foreco.2017.05.041
Scopus记录号: 2-s2.0-85019973799
论文题名: Near-infrared reflectance spectroscopy allows rapid and simultaneous evaluation of chloroplast pigments and antioxidants, carbon isotope discrimination and nitrogen content in Populus spp. leaves
作者: Fernàndez-Martínez J. ; Joffre R. ; Zacchini M. ; Fernández-Marín B. ; García-Plazaola J.I. ; Fleck I.
刊名: Forest Ecology and Management
ISSN: 0378-1127
出版年: 2017
卷: 399 起始页码: 227
结束页码: 234
语种: 英语
英文关键词: Antioxidant
; Carbon isotope discrimination
; NIRS
; Nitrogen
; Photoprotection
; Populus
Scopus关键词: Bioremediation
; Calibration
; Chemical analysis
; Conservation
; Forestry
; Infrared devices
; Infrared transmission
; Isotopes
; Least squares approximations
; Near infrared spectroscopy
; Nitrogen
; Physiological models
; Physiology
; Plants (botany)
; Reflection
; Spectroscopy
; Carbon isotope discrimination
; Environmental constraints
; Near infrared reflectance spectroscopy
; NIRS
; Nutritional characteristics
; Partial least squares regression
; Photoprotection
; Populus
; Antioxidants
; antioxidant
; bioenergy
; bioremediation
; calibration
; carbon isotope
; chloroplast
; deciduous tree
; ecophysiology
; nitrogen
; photoprotection
; Populus
英文摘要: Poplar species are economically important sources of timber and bioenergy and they also have a valuable application in phytoremediation and in forest restoration programmes. The characterization of their responses in the face of environmental constraints is essential to ascertain their adaptation capacity. Quantification of chloroplast pigments and antioxidant compounds, nutrients and carbon isotope discrimination (Δ13C) of a large set of samples in ecophysiological studies is important for determining the plant's physiological responses. Δ13C and nitrogen are parameters of special interest for providing information on photosynthesis, water relations and nutritional characteristics in forest stands. Photoprotective and antioxidant compounds participate in the physiological defence of plants subjected to stressful environmental conditions. The measurement of these leaf components by traditional techniques is laborious and expensive. To evaluate them, a calibration model was obtained using Near-Infrared Reflectance Spectroscopy (NIRS) and chemical analyses of Populus spp. leaves sampled from different clones in different years under different physiological conditions. Predictive calibration equations for the concentration of chloroplast pigments (Chlorophylls a and b, lutein, neoxanthin, β-carotene (β-Car), VAZ (violaxanthin + antheraxanthin + zeaxanthin), epoxidation index (EI = (0.5·antheraxanthin + violaxanthin)/VAZ)), antioxidants (total phenolics (TPhe), ascorbate and α-tocopherol), Δ13C and N content were established using a partial least squares regression algorithm. Results showed a remarkable accuracy of the calibration equations for quantifying several antioxidative and photoprotective defence compounds such as neoxanthin (R2 = 0.844), β-Car (R2 = 0.827), VAZ (R2 = 0.846), EI (R2 = 0.831) and excellent accuracy for TPhe (R2 = 0.957), N (R2 = 0.963) and Δ13C (R2 = 0.922). As shown, results can be obtained on multiple physiological attributes with a single measurement, saving time and money and demonstrating the suitability of NIRS for ecophysiological purposes and bioremediation programs. © 2017 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/64271
Appears in Collections: 影响、适应和脆弱性
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作者单位: Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat Biologia, Universitat de Barcelona, Diagonal 645, Barcelona, Spain; Centre National de la Recherche Scientifique CNRS, CEFE UMR 5175, CNRS – Université de Montpellier – Université Paul-Valéry Montpellier – EPHE – IRD, Montpellier, France; Istituto di Biologia Agroambientale e Forestale-CNR, Via Salaria km 29.300, 00016 Monterotondo Scalo, Rome, Italy; Departamento de Biología Vegetal y Ecología, Universidad del País Vasco, Apdo 644, Bilbao, Spain
Recommended Citation:
Fernàndez-Martínez J.,Joffre R.,Zacchini M.,et al. Near-infrared reflectance spectroscopy allows rapid and simultaneous evaluation of chloroplast pigments and antioxidants, carbon isotope discrimination and nitrogen content in Populus spp. leaves[J]. Forest Ecology and Management,2017-01-01,399