DOI: 10.1002/2013GB004690
Scopus记录号: 2-s2.0-84902024658
论文题名: Experimental assessment of diazotroph responses to elevated seawater pCO2 in the North Pacific Subtropical Gyre
作者: Böttjer D ; , Karl D ; M ; , Letelier R ; M ; , Viviani D ; A ; , Church M ; J
刊名: Global Biogeochemical Cycles
ISSN: 8866236
出版年: 2014
卷: 28, 期: 6 起始页码: 601
结束页码: 616
语种: 英语
英文关键词: CO2
; diazotrophs
; nitrogen fixation
; North Pacific Subtropical Gyre
; ocean acidification
; Station ALOHA
Scopus关键词: Analysis of variance (ANOVA)
; Carbon dioxide
; Experiments
; Gene expression
; Nitrogen fixation
; Photosynthesis
; Phytoplankton
; Plankton
; Polymerase chain reaction
; Tropics
; CO2
; Diazotrophs
; Ocean acidifications
; Station ALOHA
; Subtropical Gyre
; Seawater
; Cyanobacteria
英文摘要: We examined short-term (24-72 h) responses of naturally occurring marine N2 fixing microorganisms (termed diazotrophs) to abrupt increases in the partial pressure of carbon dioxide (pCO2) in seawater during nine incubation experiments conducted between May 2010 and September 2012 at Station ALOHA (A Long-term Oligotrophic Habitat Assessment) (22°45′N, 158°W) in the North Pacific Subtropical Gyre (NPSG). Rates of N2 fixation, nitrogenase (nifH) gene abundances and transcripts of six major groups of cyanobacterial diazotrophs (including both unicellular and filamentous phylotypes), and rates of primary productivity (as measured by 14C-bicarbonate assimilation into plankton biomass) were determined under contemporary (~390 ppm) and elevated pCO2 conditions (~1100 ppm). Quantitative polymerase chain reaction (QPCR) amplification of planktonic nifH genes revealed that unicellular cyanobacteria phylotypes dominated gene abundances during these experiments. In the majority of experiments (seven out of nine), elevated pCO2 did not significantly influence rates of dinitrogen (N2) fixation or primary productivity (two-way analysis of variance (ANOVA), P > 0.05). During two experiments, rates of N2 fixation and primary productivity were significantly lower (by 79 to 82% and 52 to 72%, respectively) in the elevated pCO2 treatments relative to the ambient controls (two-way ANOVA, P < 0.05). QPCR amplification of nifH genes and gene transcripts revealed that diazotroph abundances and nifH gene expression were largely unchanged by the perturbation of the seawater pCO 2. Our results suggest that naturally occurring N2 fixing plankton assemblages in the NPSG are relatively resilient to large, short-term increases in pCO2. ©2014. American Geophysical Union. All Rights Reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/77528
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Oceanography, University of Hawai'i at Manoa, Honolulu, HI, United States; Daniel K. Inouye Center for Microbial Oceanography: Research and Education, University of Hawai'i at Manoa, Honolulu HI, United States; College of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR, United States
Recommended Citation:
Böttjer D,, Karl D,M,et al. Experimental assessment of diazotroph responses to elevated seawater pCO2 in the North Pacific Subtropical Gyre[J]. Global Biogeochemical Cycles,2014-01-01,28(6)