DOI: 10.1016/j.atmosenv.2013.11.036
Scopus记录号: 2-s2.0-84890212369
论文题名: Combining real-time PCR and next-generation DNA sequencing to provide quantitative comparisons of fungal aerosol populations
作者: Dannemiller K ; C ; , Lang-Yona N ; , Yamamoto N ; , Rudich Y ; , Peccia J
刊名: Atmospheric Environment
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2014
卷: 84 起始页码: 113
结束页码: 121
语种: 英语
英文关键词: 454 Pyrosequencing
; Allergens
; Bioaerosols
; Fungi
; Israel
; ITS
Scopus关键词: Aerosols
; Allergens
; DNA sequences
; Metadata
; Polymerase chain reaction
; Population statistics
; 454-Pyrosequencing
; Bioaerosols
; Cladosporium cladosporioides
; Internal transcribed spacers
; Israel
; ITS
; Next-generation dna sequencing
; Principal coordinate analysis
; Fungi
; allergen
; ergosterol
; internal transcribed spacer
; aerosol
; air sampling
; comparative study
; correlation
; DNA
; fungus
; pathogen
; polymerase chain reaction
; population structure
; quantitative analysis
; real time
; species diversity
; taxonomy
; aerosol
; air sampling
; airborne fungus
; Alternaria
; Alternaria alternata
; article
; Aspergillus
; autumn
; Cladosporium
; Cladosporium cladosporioides
; Cochliobolus
; comparative study
; Cryptococcus
; Curvularia
; DNA sequence
; fungal community
; fungal genome
; fungus spore
; Fusarium
; Israel
; nonhuman
; particulate matter
; Penicillium
; principal coordinate analysis
; priority journal
; pyrosequencing
; real time polymerase chain reaction
; Rhodotorula
; spring
; Stachybotrys
; Stachybotrys chartarum
; Ustilago
; Israel
; Alternaria
; Alternaria alternata
; Aspergillus
; Cladosporium
; Cladosporium cladosporioides
; Epicoccum nigrum
; Fungi
; Fusarium
; Penicillium
; Ulocladium
Scopus学科分类: Environmental Science: Water Science and Technology
; Earth and Planetary Sciences: Earth-Surface Processes
; Environmental Science: Environmental Chemistry
英文摘要: We examined fungal communities associated with the PM10 mass of Rehovot, Israel outdoor air samples collected in the spring and fall seasons. Fungal communities were described by 454 pyrosequencing of the internal transcribed spacer (ITS) region of the fungal ribosomal RNA encoding gene. To allow for a more quantitative comparison of fungal exposure in humans, the relative abundance values of specific taxa were transformed to absolute concentrations through multiplying these values by the sample's total fungal spore concentration (derived from universal fungal qPCR). Next, the sequencing-based absolute concentrations for Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, and Penicillium/Aspergillus spp. were compared to taxon-specific qPCR concentrations for A.alternata, C.cladosporioides, E.nigrum, and Penicillium/Aspergillus spp. derived from the same spring and fall aerosol samples. Results of these comparisons showed that the absolute concentration values generated from pyrosequencing were strongly associated with the concentration values derived from taxon-specific qPCR (for all four species, p<0.005, all R>0.70). The correlation coefficients were greater for species present in higher concentrations. Our microbial aerosol population analyses demonstrated that fungal diversity (number of fungal operational taxonomic units) was higher in the spring compared to the fall (p=0.02), and principal coordinate analysis showed distinct seasonal differences in taxa distribution (ANOSIM p=0.004). Among genera containing allergenic and/or pathogenic species, the absolute concentrations of Alternaria, Aspergillus, Fusarium, and Cladosporium were greater in the fall, while Cryptococcus, Penicillium, and Ulocladium concentrations were greater in the spring. The transformation of pyrosequencing fungal population relative abundance data to absolute concentrations can improve next-generation DNA sequencing-based quantitative aerosol exposure assessment. © 2013 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/81089
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Chemical and Environmental Engineering, Yale University, Mason Laboratory, 9 Hillhouse Avenue, New Haven, CT 06520-8286, United States; Department of Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel; Department of Environmental Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea
Recommended Citation:
Dannemiller K,C,, Lang-Yona N,et al. Combining real-time PCR and next-generation DNA sequencing to provide quantitative comparisons of fungal aerosol populations[J]. Atmospheric Environment,2014-01-01,84