DOI: 10.1016/j.atmosenv.2015.09.010
Scopus记录号: 2-s2.0-84941651553
论文题名: Fractionating ambient humic-like substances (HULIS) for their reactive oxygen species activity - Assessing the importance of quinones and atmospheric aging
作者: Verma V ; , Wang Y ; , El-Afifi R ; , Fang T ; , Rowland J ; , Russell A ; G ; , Weber R ; J
刊名: Atmospheric Environment
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2015
卷: 120 起始页码: 351
结束页码: 359
语种: 英语
英文关键词: Atmospheric aging
; Dithiothreitol (DTT assay)
; Humic-like substances (HULIS)
; Quinones
; Reactive oxygen species (ROS)
Scopus关键词: Aerosols
; Dichloromethane
; Hexane
; Ketones
; Methanol
; Organic solvents
; Oxygen
; Redox reactions
; Water pollution
; 1 ,2-naphthoquinone
; 1 ,4-Naphthoquinone
; 9 ,10-Phenanthrenequinone
; Dithiothreitol
; Humic-like substances
; Oxygenated derivatives
; Quinones
; Reactive oxygen species
; Atmospheric aerosols
; 1,2 naphthoquinone
; 1,4 naphthoquinone
; dichloromethane
; dithiothreitol
; hexane
; juglone
; methanol
; phenanthrenequinone
; quinone derivative
; reactive oxygen metabolite
; aerosol
; assessment method
; atmospheric pollution
; humic substance
; methanol
; organic compound
; oxidation
; oxygen
; particulate matter
; reduction
; solubility
; air pollution
; Article
; atmospheric deposition
; environmental temperature
; filter
; humic substance
; priority journal
Scopus学科分类: Environmental Science: Water Science and Technology
; Earth and Planetary Sciences: Earth-Surface Processes
; Environmental Science: Environmental Chemistry
英文摘要: In this paper, we present a technique to identify the redox-active components of fine organic aerosols by fractionating humic-like substances (HULIS). We applied this technique to a dithiothreitol (DTT) assay - a measure of the capability of PM to generate reactive oxygen species (ROS), and assessed the contribution of quinones to the DTT activity of ambient water-soluble PM. Filter samples from the Southeastern Center for Air Pollution & Epidemiology (SCAPE) were extracted in water and then passed-through a C-18 column to isolate the HULIS fraction by retention on the column. The HULIS was then eluted with a sequence of solvents of increasing polarity, i.e., hexane, dichloromethane (DCM) and then methanol. Each of these eluted fractions was analyzed for DTT activity. The methanol fraction was found to possess most of the DTT activity (>70%), while the hexane fraction had the least activity (<5%), suggesting that the ROS-active compounds of ambient water-soluble PM2.5 HULIS are mostly polar in nature. A number of quinones thought to contribute to ambient PM DTT activity were also tested. 1,4 Naphthoquinone (1,4 NQ), 1,2 Naphthoquinone (1,2 NQ), 9,10 Phenanthrenequinone (PQ), and 5-hydroxy-1,4 NQ were analyzed by the same protocol. The hexane fraction of two quinones (PQ, and 1,4 NQ) was the most-DTT active, while methanol was the least, confirming that PQ, 1,4 NQ, and 1,2 NQ (which could not be recovered from the column) do not contribute significantly to the water-soluble DTT activity of ambient PM2.5. However, an oxygenated derivative of 1,4 NQ, (5-hydroxy-1,4 NQ), which is also intrinsically more DTT-active than 1,4 NQ, was mostly (>60%) eluted in methanol. The results demonstrate the importance of atmospheric aging (oxidation) of organic aerosols in enhancing the ROS activity of ambient PM. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/81474
Appears in Collections: 气候变化事实与影响
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作者单位: Georgia Institute of Technology, Atlanta, GA, United States
Recommended Citation:
Verma V,, Wang Y,, El-Afifi R,et al. Fractionating ambient humic-like substances (HULIS) for their reactive oxygen species activity - Assessing the importance of quinones and atmospheric aging[J]. Atmospheric Environment,2015-01-01,120