DOI: 10.1007/s10533-017-0343-8
Scopus记录号: 2-s2.0-85020124705
论文题名: Carbon quantity and quality drives variation in cave microbial communities and regulates Mn(II) oxidation
作者: Cloutier M.L.C. ; Carmichael S.K. ; Carson M.A. ; Madritch M.D. ; Bräuer S.L.
刊名: Biogeochemistry
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2017
卷: 134, 期: 2018-01-02 起始页码: 77
结束页码: 94
语种: 英语
英文关键词: Bacterial communities
; Caves
; Enzyme
; Exogenous carbon
; Fungal communities
; Manganese oxidation
Scopus关键词: anthropogenic effect
; bacterium
; cave
; community structure
; enzyme
; enzyme activity
; fungus
; manganese
; microbial community
; oxidation
; soil carbon
; Appalachians
; United States
; Archaea
; Bacteria (microorganisms)
; Basidiomycota
; Zygomycetes
英文摘要: Cave ecosystems are carbon limited and thus are particularly susceptible to anthropogenic pollution. Yet, how carbon quality and quantity that can modulate the pathways and amount of Mn cycling in caves remains largely unknown. To explore Mn cycling, baseline bacterial, archaeal, and fungal communities associated with Mn(III/IV) oxide deposits were assessed in both relatively ‘pristine’ and anthropogenically impacted caves in the Appalachian Mountains (USA). Cave sites were then amended with various carbon sources that are commonly associated with anthropogenic input to determine whether they stimulate biotic Mn(II) oxidation in situ. Results revealed patterns between sites that had long-term exogenous carbon loading compared to sites that were relatively ‘pristine,’ particularly among Bacteria and Archaea. Carbon treatments that stimulated Mn(II) oxidation at several sites resulted in significant changes to the microbial communities, indicating that anthropogenic input can not only enhance biotic Mn(II) oxidation, but also shape community structure and diversity. Additional carbon sources amended with copper were incubated at various cave sites to test the role that Cu(II) plays in in situ biotic Mn(II) oxidation. Media supplemented with 100 µM Cu(II) inhibited bacterial Mn(II) oxidation but stimulated fungal Mn(II) oxidation, implicating fungal use of multicopper oxidase (MCO) enzymes but bacterial use of superoxide to oxidize Mn(II). In sites with low C:N ratios, the activity of the Mn(II) oxidizing enzyme manganese peroxidase (MnP) appears to be limited (particularly by MnP-utilizing Basidiomycetes and/or Zygomycetes). © 2017, Springer International Publishing Switzerland. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/83259
Appears in Collections: 气候减缓与适应 气候变化事实与影响
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作者单位: Department of Biology, Appalachian State University, 572 Rivers Street, Boone, NC, United States; Department of Ecosystem Science and Management, Pennsylvania State University, 117 Forest Resources Building, University Park, PA, United States; Department of Geology, Appalachian State University, 572 Rivers Street, Boone, NC, United States; Department of Biology, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON, United States; The Vale Living with Lakes Centre, 935 Ramsey Lake Road, Sudbury, ON, United States
Recommended Citation:
Cloutier M.L.C.,Carmichael S.K.,Carson M.A.,et al. Carbon quantity and quality drives variation in cave microbial communities and regulates Mn(II) oxidation[J]. Biogeochemistry,2017-01-01,134(2018-01-02)