DOI: 10.1007/s10533-015-0095-2
Scopus记录号: 2-s2.0-84930271156
论文题名: The effect of temperature on the rate, affinity, and 15N fractionation of NO3 − during biological denitrification in soils
作者: Maggi F. ; Riley W.J.
刊名: Biogeochemistry
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2015
卷: 124, 期: 2018-01-03 起始页码: 235
结束页码: 253
语种: 英语
英文关键词: 14N and 15N
; Affinity
; Arrhenius
; Denitrification
; Kinetic isotopic effects
; Temperature
; Transition-state theory
Scopus关键词: activation energy
; concentration (composition)
; denitrification
; enzyme
; isotopic analysis
; nitrate
; reaction kinetics
; temperature effect
; thermodynamics
英文摘要: Nine independent experiments of NO3 − denitrification were analysed using the Arrhenius law and the Eyring’s transition-state theory to highlight how temperature affects reaction rate constants, affinities, and kinetic isotopic effects. For temperatures between 20 and 35 °C, the Arrhenius law and the transition-state theory described equally well observed temperature increases in 14NO3 − and 15NO3 −denitrification rates (R > 0.99 and residuals NRMSE < 3.39 %, p < 0.01). These increases were partly caused by an increase in frequency factor and a slight decrease in activation energy (enthalpy and entropy). Parametric analysis also showed that the affinity of 14NO3 − and 15NO3 − toward a microbial enzyme increased exponentially with temperature and a strong correlation with the rate constants was found (R = 0.93, p < 0.01). Experimental time- and temperature-averaged fractionation factor αP/S showed only a slight increase with increasing temperature (i.e. lower isotopic effects); however, a comprehensive sensitivity analysis in the concentration-temperature domain using average thermodynamic quantities estimated here showed a more complex response; αP/S was relatively constant for initial bulk concentrations [NO3 −]0 ≤ 0.01 mol kg−1, while substantial nonlinearities developed for [NO3 −]0 ≥ 0.01 mol kg−1 and appeared to be strongly correlated with microbial biomass, whose concentration and activity varied primarily as a function of temperature and available substrate. Values of αP/S ranging between 0.9 and 0.98 for the tested temperatures suggested that interpretations of environmental isotopic signatures should include a sensitivity analysis to the temperature as this affects directly the rate constants and affinities in biochemical reactions and may hide process- and source-related isotopic effects. © 2015, Springer International Publishing Switzerland. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/83516
Appears in Collections: 气候减缓与适应 气候变化事实与影响
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作者单位: Laboratory for Advanced Environmental Engineering Research, School of Civil Engineering, The University of Sydney, Sydney, NSW, Australia; Earth Systems Division, Climate and Carbon Department, Lawrence Berkeley National Laboratory, Berkeley, CA, United States
Recommended Citation:
Maggi F.,Riley W.J.. The effect of temperature on the rate, affinity, and 15N fractionation of NO3 − during biological denitrification in soils[J]. Biogeochemistry,2015-01-01,124(2018-01-03)