DOI: 10.1111/gbi.12166
Scopus记录号: 2-s2.0-85027939183
论文题名: The influence of hydrogeological disturbance and mining on coal seam microbial communities
作者: Raudsepp M.J. ; Gagen E.J. ; Evans P. ; Tyson G.W. ; Golding S.D. ; Southam G.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2016
卷: 14, 期: 2 起始页码: 163
结束页码: 175
语种: 英语
Scopus关键词: archaeal DNA
; bacterial DNA
; coal
; methane
; ribosome DNA
; RNA 16S
; anoxic conditions
; biomass
; coal mine
; coal seam
; disturbance
; groundwater flow
; hydrogeology
; methane
; methanogenesis
; methanotrophy
; microbial community
; nitrate
; stable isotope
; archaeon
; bacterium
; biota
; chemistry
; classification
; cluster analysis
; DNA sequence
; genetics
; isolation and purification
; metabolism
; microbiology
; mining
; phylogeny
; Queensland
; Archaea
; Bacteria
; Biota
; Cluster Analysis
; Coal
; DNA, Archaeal
; DNA, Bacterial
; DNA, Ribosomal
; Methane
; Mining
; Phylogeny
; Queensland
; RNA, Ribosomal, 16S
; Sequence Analysis, DNA
; Soil Microbiology
; Australia
; Bowen Basin
; Queensland
; Archaea
; Bacteria (microorganisms)
; Methanobacteriaceae
; Methanococcaceae
; Methanosaeta
; Pseudomonas
; Rhodocyclaceae
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: The microbial communities present in two underground coal mines in the Bowen Basin, Queensland, Australia, were investigated to deduce the effect of pumping and mining on subsurface methanogens and methanotrophs. The micro-organisms in pumped water from the actively mined areas, as well as, pre- and post-mining formation waters were analyzed using 16S rRNA gene amplicon sequencing. The methane stable isotope composition of Bowen Basin coal seam indicates that methanogenesis has occurred in the geological past. More recently at the mine site, changing groundwater flow dynamics and the introduction of oxygen in the subsurface has increased microbial biomass and diversity. Consistent with microbial communities found in other coal seam environments, pumped coal mine waters from the subsurface were dominated by bacteria belonging to the genera Pseudomonas and the family Rhodocyclaceae. These environments and bacterial communities supported a methanogen population, including Methanobacteriaceae, Methanococcaceae and Methanosaeta. However, one of the most ubiquitous micro-organisms in anoxic coal mine waters belonged to the family 'Candidatus Methanoperedenaceae'. As the Archaeal family 'Candidatus Methanoperedenaceae' has not been extensively defined, the one studied species in the family is capable of anaerobic methane oxidation coupled to nitrate reduction. This introduces the possibility that a methane cycle between archaeal methanogenesis and methanotrophy may exist in the anoxic waters of the coal seam after hydrogeological disturbance. © 2015 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85112
Appears in Collections: 影响、适应和脆弱性
There are no files associated with this item.
作者单位: School of Earth Sciences, The University of Queensland, St. Lucia, Qld, Australia; Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Qld, Australia; Advanced Water Management Centre, The University of Queensland, St. Lucia, Qld, Australia
Recommended Citation:
Raudsepp M.J.,Gagen E.J.,Evans P.,et al. The influence of hydrogeological disturbance and mining on coal seam microbial communities[J]. Geobiology,2016-01-01,14(2)