globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gbi.12162
Scopus记录号: 2-s2.0-84955655616
论文题名:
Dominance of 'Gallionella capsiferriformans' and heavy metal association with Gallionella-like stalks in metal-rich pH 6 mine water discharge
作者: Fabisch M.; Freyer G.; Johnson C.A.; Büchel G.; Akob D.M.; Neu T.R.; Küsel K.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2016
卷: 14, 期:1
起始页码: 68
结束页码: 90
语种: 英语
Scopus关键词: bacterium ; freshwater environment ; groundwater pollution ; heavy metal ; iron oxide ; mine drainage ; oxidation ; pH ; sediment pollution ; streamwater ; uranium ; Germany ; Ronneburg ; Thuringia ; Gallionella ; Gallionella ferruginea ; Gallionellaceae ; Streptopus ; bacterial DNA ; ferric ion ; ferric oxide ; heavy metal ; iron ; ribosome DNA ; RNA 16S ; water ; aerobic metabolism ; biota ; chemistry ; classification ; confocal microscopy ; DNA sequence ; Gallionellaceae ; genetics ; Germany ; isolation and purification ; metabolism ; microbiology ; molecular genetics ; oxidation reduction reaction ; pH ; scanning electrochemical microscopy ; transmission electron microscopy ; Aerobiosis ; Biota ; DNA, Bacterial ; DNA, Ribosomal ; Ferric Compounds ; Gallionellaceae ; Germany ; Hydrogen-Ion Concentration ; Iron ; Metals, Heavy ; Microscopy, Confocal ; Microscopy, Electrochemical, Scanning ; Microscopy, Electron, Transmission ; Molecular Sequence Data ; Oxidation-Reduction ; RNA, Ribosomal, 16S ; Sequence Analysis, DNA ; Water ; Water Microbiology
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences ; Environmental Science: General Environmental Science ; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Heavy metal-contaminated, pH 6 mine water discharge created new streams and iron-rich terraces at a creek bank in a former uranium-mining area near Ronneburg, Germany. The transition from microoxic groundwater with ∼5 mm Fe(II) to oxic surface water may provide a suitable habitat for microaerobic iron-oxidizing bacteria (FeOB). In this study, we investigated the potential contribution of these FeOB to iron oxidation and metal retention in this high-metal environment. We (i) identified and quantified FeOB in water and sediment at the outflow, terraces, and creek, (ii) studied the composition of biogenic iron oxides (Gallionella-like twisted stalks) with scanning and transmission electron microscopy (SEM, TEM) as well as confocal laser scanning microscopy (CLSM), and (iii) examined the metal distribution in sediments. Using quantitative PCR, a very high abundance of FeOB was demonstrated at all sites over a 6-month study period. Gallionella spp. clearly dominated the communities, accounting for up to 88% of Bacteria, with a minor contribution of other FeOB such as Sideroxydans spp. and 'Ferrovum myxofaciens'. Classical 16S rRNA gene cloning showed that 96% of the Gallionella-related sequences had ≥97% identity to the putatively metal-tolerant 'Gallionella capsiferriformans ES-2', in addition to known stalk formers such as Gallionella ferruginea and Gallionellaceae strain R-1. Twisted stalks from glass slides incubated in water and sediment were composed of the Fe(III) oxyhydroxide ferrihydrite, as well as polysaccharides. SEM and scanning TEM-energy-dispersive X-ray spectroscopy revealed that stalk material contained Cu and Sn, demonstrating the association of heavy metals with biogenic iron oxides and the potential for metal retention by these stalks. Sequential extraction of sediments suggested that Cu (52-61% of total sediment Cu) and other heavy metals were primarily bound to the iron oxide fractions. These results show the importance of 'G. capsiferriformans' and biogenic iron oxides in slightly acidic but highly metal-contaminated freshwater environments. Copyright © 2016 John Wiley & Sons Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85121
Appears in Collections:影响、适应和脆弱性

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作者单位: Institute of Ecology, Friedrich Schiller University Jena, Jena, Germany; Department of Geosciences, Virginia Tech, Blacksburg, VA, United States; Institute of Geosciences, Friedrich Schiller University Jena, Jena, Germany; U.S. Geological Survey, Reston, VA, United States; Department of River Ecology, Helmholtz Centre for Environmental Research Leipzig-Halle - UFZ, Magdeburg, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany

Recommended Citation:
Fabisch M.,Freyer G.,Johnson C.A.,et al. Dominance of 'Gallionella capsiferriformans' and heavy metal association with Gallionella-like stalks in metal-rich pH 6 mine water discharge[J]. Geobiology,2016-01-01,14(1)
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