DOI: 10.1111/gbi.12152
Scopus记录号: 2-s2.0-84944178697
论文题名: Indigenous arsenic(V)-reducing microbial communities in redox-fluctuating near-surface sediments of the Mekong Delta
作者: Ying S.C. ; Damashek J. ; Fendorf S. ; Francis C.A.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2015
卷: 13, 期: 6 起始页码: 581
结束页码: 587
语种: 英语
Scopus关键词: anoxic conditions
; arsenate
; arsenic
; community structure
; enzyme activity
; gene expression
; genetic marker
; microbial community
; redox conditions
; reduction
; seasonal variation
; species diversity
; Cambodia
; Mekong Delta
; Viet Nam
; Geobacter sp.
; Otus
; arsenic
; bacterium
; Cambodia
; classification
; DNA sequence
; genetics
; isolation and purification
; metabolism
; microbiology
; oxidation reduction reaction
; river
; sediment
; Arsenic
; Bacteria
; Cambodia
; Geologic Sediments
; Oxidation-Reduction
; Rivers
; Sequence Analysis, DNA
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Arsenic (As) cycling within soils and sediments of the Mekong Delta of Cambodia is affected by drastic redox fluctuations caused by seasonal monsoons. Extensive flooding during monsoon seasons creates anoxic soil conditions that favor anaerobic microbial processes, including arsenate [As(V)] respiration-a process contributing to the mobilization of As. Repeated oxidation and reduction in near-surface sediments, which contain 10-40 mg kg-1 As, lead to the eventual downward movement of As to the underlying aquifer. Amplification of a highly conserved functional gene encoding dissimilatory As(V) reductase, arrA, can be used as a molecular marker to detect the genetic potential for As(V) respiration in environmental samples. However, few studies have successfully amplified arrA from sediments without prior enrichment, which can drastically shift community structure. In the present study, we examine the distribution and diversity of arrA genes amplified from multiple sites within the Cambodian Mekong Delta as a function of near-surface depth (10, 50, 100, 200, and 400 cm), where sediments undergo seasonal redox fluctuations. We report successful amplification of 302 arrA gene sequences (72 OTUs) from near-surface Cambodian soils (without prior enrichment or stimulation with carbon amendments), where a large majority (>70%) formed a well-supported clade that is phylogenetically distinct from previously reported sequences from Cambodia and other South and Southeast Asian sediments, with highest sequence similarity to known Geobacter species capable of As(V) respiration, further supporting the potentially important role of Geobacter sp. in arsenic mobilization in these regions. © 2015 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85123
Appears in Collections: 影响、适应和脆弱性
There are no files associated with this item.
作者单位: Department of Environmental Sciences, University of California-Riverside, Riverside, CA, United States; Earth System Science Department, Stanford University, Stanford, CA, United States
Recommended Citation:
Ying S.C.,Damashek J.,Fendorf S.,et al. Indigenous arsenic(V)-reducing microbial communities in redox-fluctuating near-surface sediments of the Mekong Delta[J]. Geobiology,2015-01-01,13(6)