DOI: 10.1111/gbi.12135
Scopus记录号: 2-s2.0-84926646979
论文题名: Pigment production and isotopic fractionations in continuous culture: Okenone producing purple sulfur bacteria Part II
作者: Smith D.A. ; Steele A. ; Fogel M.L.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2015
卷: 13, 期: 3 起始页码: 292
结束页码: 301
语种: 英语
Scopus关键词: biomass
; carotenoid
; diagenesis
; euxinic environment
; fossil record
; isotopic fractionation
; light intensity
; micropaleontology
; pigment
; sulfate-reducing bacterium
; Bacteria (microorganisms)
; Chromatiaceae
; Marichromatium purpuratum
; bacteriochlorophyll
; carbon
; carotenoid
; okenone
; biosynthesis
; chemistry
; Chromatiaceae
; metabolism
; Bacteriochlorophylls
; Carbon Isotopes
; Carotenoids
; Chromatiaceae
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Okenone is a carotenoid pigment unique to certain members of Chromatiaceae, the dominant family of purple sulfur bacteria (PSB) found in euxinic photic zones. Diagenetic alteration of okenone produces okenane, the only recognized molecular fossil unique to PSB. The in vivo concentrations of okenone and bacteriochlorophyll a (Bchl a) on a per cell basis were monitored and quantified as a function of light intensity in continuous cultures of the purple sulfur bacterium Marichromatium purpuratum (Mpurp1591). We show that okenone-producing PSB have constant bacteriochlorophyll to carotenoid ratios in light-harvesting antenna complexes. The in vivo concentrations of Bchl a, 0.151 ± 0.012 fmol cell-1, and okenone, 0.103 ± 0.012 fmol cell-1, were not dependent on average light intensity (10-225 Lux) at both steady and non-steady states. This observation revealed that in autotrophic continuous cultures of Mpurp1591, there was a constant ratio for okenone to Bchl a of 1:1.5. Okenone was therefore constitutively produced in planktonic cultures of PSB, regardless of light intensity. This confirms the legitimacy of okenone as a signature for autotrophic planktonic PSB and by extrapolation water column euxinia. We measured the δ13C, δ15N, and δ34S bulk biomass values from cells collected daily and determined the isotopic fractionations of Mpurp1591. There was no statistical relationship in the bulk isotope measurements or stable isotope fractionations to light intensity or cell density under steady and non-steady-state conditions. The carbon isotope fractionation between okenone and Bchl a with respect to overall bulk biomass (13εpigment - biomass ) was 2.2 ± 0.4‰ and -4.1 ± 0.9‰, respectively. The carbon isotopic fractionation (13εpigment-CO2) for the production of pigments in PSB is more variable than previously thought with our reported values for okenone at -15.5 ± 1.2‰ and -21.8 ± 1.7‰ for Bchl a. © 2015 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85157
Appears in Collections: 影响、适应和脆弱性
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作者单位: Department of Earth Sciences, Dartmouth College, Hanover, NH, United States; Geophysical Laboratory of the Carnegie Institution of Washington, Washington, DC, United States; School of Natural Sciences, University of California, Merced, Merced, CA, United States; Division of Geological and Planetary Sciences, California Institute of Technology, MC 100-23, 1200 East California Boulevard, Pasadena, CA, United States
Recommended Citation:
Smith D.A.,Steele A.,Fogel M.L.. Pigment production and isotopic fractionations in continuous culture: Okenone producing purple sulfur bacteria Part II[J]. Geobiology,2015-01-01,13(3)