DOI: 10.1111/gbi.12065
Scopus记录号: 2-s2.0-84890240987
论文题名: Deep subsurface sulfate reduction and methanogenesis in the Iberian Pyrite Belt revealed through geochemistry and molecular biomarkers
作者: Puente-Sánchez F. ; Moreno-Paz M. ; Rivas L.A. ; Cruz-Gil P. ; García-Villadangos M. ; Gómez M.J. ; Postigo M. ; Garrido P. ; González-Toril E. ; Briones C. ; Fernández-Remolar D. ; Stoker C. ; Amils R. ; Parro V.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2014
卷: 12, 期: 1 起始页码: 34
结束页码: 47
语种: 英语
Scopus关键词: antibody
; borehole
; geochemistry
; geomicrobiology
; hybridization
; massive sulfide
; methane
; methanogenesis
; microbial community
; molecular ecology
; oxidation
; pyrite
; sulfate
; sulfur
; Iberian Pyrite Belt
; Bacteria (microorganisms)
; Euryarchaeota
; Posibacteria
; Prokaryota
; methane
; sulfate
; article
; bacterium
; biota
; chemistry
; classification
; DNA microarray
; Euryarchaeota
; fluorescence in situ hybridization
; genetics
; immunology
; metabolism
; microarray analysis
; microbiology
; oxidation reduction reaction
; protein microarray
; soil
; Spain
; Bacteria
; Biota
; Euryarchaeota
; In Situ Hybridization, Fluorescence
; Methane
; Microarray Analysis
; Oligonucleotide Array Sequence Analysis
; Oxidation-Reduction
; Protein Array Analysis
; Soil
; Soil Microbiology
; Spain
; Sulfates
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: The Iberian Pyrite Belt (IPB, southwest of Spain), the largest known massive sulfide deposit, fuels a rich chemolithotrophic microbial community in the Río Tinto area. However, the geomicrobiology of its deep subsurface is still unexplored. Herein, we report on the geochemistry and prokaryotic diversity in the subsurface (down to a depth of 166 m) of the Iberian Pyritic belt using an array of geochemical and complementary molecular ecology techniques. Using an antibody microarray, we detected polymeric biomarkers (lipoteichoic acids and peptidoglycan) from Gram-positive bacteria throughout the borehole. DNA microarray hybridization confirmed the presence of members of methane oxidizers, sulfate-reducers, metal and sulfur oxidizers, and methanogenic Euryarchaeota. DNA sequences from denitrifying and hydrogenotrophic bacteria were also identified. FISH hybridization revealed live bacterial clusters associated with microniches on mineral surfaces. These results, together with measures of the geochemical parameters in the borehole, allowed us to create a preliminary scheme of the biogeochemical processes that could be operating in the deep subsurface of the Iberian Pyrite Belt, including microbial metabolisms such as sulfate reduction, methanogenesis and anaerobic methane oxidation. © 2013 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85169
Appears in Collections: 影响、适应和脆弱性
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作者单位: Departments of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Madrid, Spain; Planetology and Habitability, Centro de Astrobiología (INTA-CSIC), Madrid, Spain; NASA Ames Research Center, Moffett Field, CA, United States; Centro de Biología Molecular Severo Oochoa (UAM-CSIC), Cantoblanco, Madrid, Spain
Recommended Citation:
Puente-Sánchez F.,Moreno-Paz M.,Rivas L.A.,et al. Deep subsurface sulfate reduction and methanogenesis in the Iberian Pyrite Belt revealed through geochemistry and molecular biomarkers[J]. Geobiology,2014-01-01,12(1)